OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-S100A4 antibodies

Anti-S100A4 Antibody EPR2761(2)

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310834
  • Rabbit Monoclonal antibody against S100A4
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC418991) , 20ug Explanation
100ul 325 In Stock
Add to Shopping Cart
WB(1)
IHC(2)
IF(1)
FC(1)
spacer
Also for S100A4 (NM_002961)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the C-terminus in human S100A4 was used as an immunogen.
Clone NameEPR2761(2) IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsICC/IF: 1:100 - 1:250; WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:250 - 1:500; FC: 1:1000
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens S100 calcium binding protein A4 (S100A4), transcript variant 1
Alternative Name18A2; 42A; CAPL; FSP1; MTS1; P9KA; PEL98
Database LinkNP_002952
Entrez Gene 6275 Human
FunctionS100A4 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and they are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 proteins include at least 13 members. S100A4 may function in motility, invasion, and tubulin polymerization. Altered expression of S100A4 have been implicated in tumor metastasis.
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-S100A4 antibody [EPR2761(2)]; All lanes : Anti-S100A4 antibody [EPR2761(2)] at 1/1000 dilution.Lane 1 : Human tonsil lysate.Lane 2 : A549 cell lysate.Lane 3 : A375 cell lysate.Lane 4 : Human small intestine lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 12 kDa.Observed band size : 12 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR2761(2)]; TA310834, at 1/250 dilution, staining S100A4 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR2761(2)]; Immunohistochemical analysis of Human lung tissue, staining S100A4 with TA310834.Tissue was fixed with HOPE and blocked with blocking solution for 5 minutes at 25°C. Samples were incubated with primary antibody (1/1000 in diluent) for 1 hour at 25°C. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-S100A4 antibody [EPR2761(2)]; TA310834 staining S100A4 in the A549 cell line from Human lungs by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with Triton X-100 0.25% in PBS. Samples were incubated with primary antibody (1/100) for45 minutesat 25°C. A TRITC-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.
FC Image
Flow Cytometry - Anti-S100A4 antibody [EPR2761(2)]; Overlay histogram showing HeLa cells stained with TA310834 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title