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Home Antibody All anti-CCNB1 antibodies

Anti-CCNB1 PHOSPHO Antibody EPR3670

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310251
  • Rabbit Monoclonal antibody against Cyclin B1, Ps126 (CCNB1) (Phospho-specific)
100ul $325 3-7 Days
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WB(1)

OriGene Data

ImmunogenA phospho specific peptide corresponding to residues surrounding Serine 126 of human Cyclin B1 was used as an immunogen. This antibody only detects Cyclin B1 phosphorylated at Serine 126.
Clone NameEPR3670 IsotypeTissue culture supernatant
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
Purification pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Note Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

Reference Data

Target NameHomo sapiens cyclin B1 (CCNB1)
Alternative NameCCNB
Database LinkNP_114172
FunctionCyclin B1 regulates mitosis in conjunction with Cdc2 kinase to form the Cdc2-cyclin B1 complex also known as maturation promoting factor (MPF) (1-2). The activation of the complex begins when the regulatory subunit, cyclin B1, associates with the catalytic subunit of Cdc2. Cyclin B1 levels rise during S phase and G2, and peak at mitosis. The timing of mitosis can also be influenced by changes in cyclin B1Cdc2 localization. Cyclin B1 is mostly located in the cytoplasm during S and G2 phases and then rapidly accumulates in the nucleus at the onset of prophase (3).
Related Pathway
Cell cycle
Hedgehog signaling pathway

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WB Image
Western blot - Anti-Cyclin B1 (phospho S126) antibody [EPR3670]; All lanes : Anti-Cyclin B1 (phospho S126) antibody [EPR3670] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : Lysate of HeLa cells treated with Nocodazole .Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution.Predicted band size : 48 kDa.

 

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