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Anti-CD3G Antibody EPR4516
Also for CD3G (NM_000073)
|A synthetic peptide corresponding to residues near the N-terminus of human CD3 gamma was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC: 1:500 - 1:1000
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens CD3g molecule, gamma (CD3-TCR complex) (CD3G)|
|CD3 is composed of five invariant polypeptide chains that associate to form three dimers. The five invariant chains of CD3 are labeled gamma, delta, epsilon, zeta, and eta (3). CD3 forms complex with the T cell antigen receptor (TCR) (1). Antigen binding to the TCR leads to IL-2 secretion via activation of a tyrosine phosphorylation pathway and a phospholipase C (PLC) pathway, in turn activating protein kinase C (1,2). Defects in this protein are associated with T cell immunodeficiency (4).|
Hematopoietic cell lineage
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Western blot - CD3G antibody [EPR4516]; All lanes : Anti-CD3G antibody [EPR4516] at 1/1000 dilution.Lane 1 : Human tonsil lysate.Lane 2 : Human fetal thymus lysate.Lane 3 : HuT-78 cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 20 kDa.
Western blot - CD3G antibody [EPR4516]; Anti-CD3G antibody [EPR4516] at 1/1000 dilution + Jurkat cell lysate at 10 µg.Predicted band size : 20 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD3G antibody [EPR4516]; ab108996, at 1/100 dilution, staining CD3G in paraffin-embedded Human tonsil tissue by Immunohistochemistry.