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Anti-ACACB PHOSPHO Antibody EPR3143Y
Also for ACACB (NM_001093)
|A synthetic peptide corresponding to residues surrounding serine 221 of human ACC2 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IP: 1:10; IHC-P: 1:100 - 1:250; ICC: 1:100
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens acetyl-CoA carboxylase beta (ACACB)|
|ACC2; ACCB; HACC275|
| Acetyl-CoA carboxylase 2 (ACC2) is a biotin-dependent enzyme that is involved in the regulation of fatty acid oxidation, rather than fatty acid biosynthesis. ACC2 is predominantly expressed in the heart, skeletal muscles and liver (1). It catalyzes the irreversible carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase and carboxyltransferase. ACC2 is throught to control fatty acid oxidation by the means of the ability of malonyl-CoA to inhibit carnitine-palmitoyl-CoA transferase I (CPT-1), the rate-limiting step in fatty acid uptake and oxidation by mitochondria (2). The activity of ACC2 is controlled by reversible phosphorylation. Ser219 and Ser221 were found to be critical for the phosphorylation and subsequent inactivation of ACC2 (3).|
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Western blot - Acetyl Coenzyme A Carboxylase (phospho S221) antibody [EPR3143Y]; All lanes : Anti-Acetyl Coenzyme A Carboxylase (phospho S221) antibody [EPR3143Y] at 1/5000 dilution.Lane 1 : HeLa cell lysate, untreated.Lane 2 : HeLa cell lysate treated with calyculin A.Lysates/proteins at 10 µg per lane.Secondary.HRP-labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 277 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Acetyl Coenzyme A Carboxylase (phospho S221) antibody [EPR3143Y]; ab109540, at 1/100 dilution, staining paraffin-embedded Human muscles by Immunohistochemistry.