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Anti-CSNK2B Antibody EP1995Y
Also for CSNK2B (NM_001320)
|A synthetic peptide corresponding to residues near the C-terminal of human CKII beta protein was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||FC: 1:20, ICC: 1:100, IHC: 1:100, WB: 1:500 - 2000,
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens casein kinase 2, beta polypeptide (CSNK2B), transcript variant 1|
|CK2B; CK2N; CSK2B; G5A|
|Casein Kinase II (CKII, CK2, phosvitin kinase, troponin T kinase, casein kinase G) is a ubiquitous serine/threonine protein kinase that is highly conserved and commonly found in eukaryotic cells (found in the cytoplasm and the nucleus). CKII is a heterotetramer that consists of two catalytic subunits (alpha, alpha-prime) and two regulatory beta subunits (1). CKII can utilize both GTP and ATP as the phosphate donor and phosphorylates threonine and serine residues. Therefore, CKII is a multifunctional protein kinase with broad range of substrates (e.g. c-Myc, c-Myb, c-Fos, c-Jun, p53, Rb, HPVE7) and it has been implicated in various cell functions/processes from cell transformation to mitosis. (2) |
Wnt Signaling Pathway
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Western blot - Casein Kinase 2 beta antibody [EP1995Y]; Anti-Casein Kinase 2 beta antibody [EP1995Y] at 1/1000 dilution + K562 cell lysate at 10 µg.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 25 kDa.Observed band size : 25 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Casein Kinase 2 beta antibody [EP1995Y]; Immunohistochemical analysis of Casein Kinase 2 beta in paraffin embedded human ovary carcinoma tissue using ab76025 at a 1/100 dilution.
Immunocytochemistry - Casein Kinase 2 beta antibody [EP1995Y]; Immunofluorescent staining of Casein Kinase 2 beta in HeLa cells using ab76025 at a 1/100 dilution.
Flow Cytometry-Anti-Casein Kinase 2 beta antibody [EP1995Y](ab76025); Overlay histogram showing HeLa cells stained with ab76025 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.