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Anti-HLA Antibody EP1395Y
|A synthetic peptide corresponding to residues in human MHC class 1 was used as an immunogen|
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||IHC-Fr: Use at an assay dependent concentration; WB: 1:10000 - 1:50000; IP: 1:30; FC: 1:10 - 1:100; IHC-P: 1:250 - 1:500; ICC/IF: 1:250 - 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Does not react with Mouse, Rat
|Homo sapiens major histocompatibility complex, class I, A (HLA-A), transcript variant 1 (A*03:01:0:01 allele)|
Entrez Gene 3105 Human
|HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq]. |
|Transmembrane EndocytosisCell adhesion molecules (CAMs)Antigen processing and presentationNatural killer cell mediated cytotoxicityType I diabetes mellitusAutoimmune thyroid diseaseMore Pathways >> |
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Western blot - Anti-HLA A [EP1395Y] antibody; Anti-HLA A [EP1395Y] antibody at 1/10000 dilution + Raji cell lysate at 10 ug.Secondary.Goat anti rabbit IgG HRP conjugated at 1/2000 dilution.Predicted band size : 41 kDa.Observed band size : 41 kDa.
Anti-HLA A [EP1395Y] antibody; Ab52922 at 1/250 dilution staining human tonsil; paraffin embedded.
ICC/IF image of TA303840 stained MCF7 cells. The cells were incubated with the antibody overnight at 4.. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at 1:1000 for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at 1:200 for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
Flow Cytometry - Anti-HLA Aantibody; Overlay histogram showing Raji cells stained with TA303840 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. This antibody gave a positive signal in Raji cells under the same conditions.