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Home Antibody All anti-HSD17B1 antibodies

Anti-HSD17B1 Antibody EP1682Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303833
  • Rabbit monoclonal antibody against HSD17B1 (C-term) (EP1682Y )
100ul 325 3-7 Days
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WB(1)
IHC(1)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the C-terminus of human HSD17B1 was used as an immunogen.
Clone NameEP1682Y IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:10000 - 1:50000; ICC/IF: 1:100 - 1:250; FC: Use 1?g for 106<:sup> cells; IP: 1:100; IHC-P: 1:100 - 1:250
Predicted MW Explanation 35 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens hydroxysteroid (17-beta) dehydrogenase 1 (HSD17B1)
Alternative NameEDH17B2; EDHB17; HSD17; SDR28C1
Database LinkNP_000404
Entrez Gene 3292 Human
Function
Related PathwayDruggable Genome Androgen and estrogen metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - HSD17B1 antibody [EP1682Y]; Anti-HSD17B1 antibody [EP1682Y] at 1/100000 dilution + Human placenta lysate at 10 ug.Secondary.Goat anti-Rabbit HRP labeled. at 1/2000 dilution.Predicted band size : 35 kDa.Observed band size : 35 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - HSD17B1 antibody [EP1682Y]; Ab51045 (1/100 dilution) staining human HSD17B1 in paraffin embedded human placenta tissue by Immunohistochemistry.
FC Image
Flow Cytometry - Anti-HSD17B1 antibody [EP1682Y]; Overlay histogram showing JEG3 cells stained with TA303833 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells ) used under the same conditions. Acquisition of >5,000 events was performed.

 

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