OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-ROCK1 antibodies

Anti-ROCK1 Antibody EP786Y

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303688
  • Rabbit monoclonal antibody against ROCK1(EP786Y)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC417325) , 20ug
100ul $325 3-7 Days
Add to Shopping Cart
WB(1)
IHC(1)
IF(1)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near C-terminus of human ROCK1 was used as an immunogen. This antibody recognizes both the cleaved C-terminus of ROCK-1 (30 kDa) and full length protein.
Clone NameEP786Y IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsIHC-Fr: 1:100; WB: 1:500; IHC-P: 1:50 - 1:100; ICC/IF: 1:250 - 1:500; IP: 1:50; FC: 1:1000
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens Rho-associated, coiled-coil containing protein kinase 1 (ROCK1)
Alternative NameP160ROCK; ROCK-I
Database LinkNP_005397
FunctionThis gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq].
Related Pathway
Focal Adhesion
Signaling by GPCR
TGF Beta Signaling Pathway
Wnt Signaling Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - ROCK1 antibody [EP786Y]; All lanes : Anti-ROCK1 antibody [EP786Y] at 1/500 dilution.Lane 1 : Untreated HeLa cell lysate.Lane 2 : Camptothecin treated HeLa lysate.Predicted band size : 158 kDa.Observed band size : 30,158 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - ROCK1 antibody [EP786Y]; Ab45171 staining human ROCK1 in human thyroid gland carcinoma by immunohistochemistry using paraffin embedded tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - ROCK1 antibody [EP786Y]; Immunofluorescent staining of HeLa cells using TA303688.
FC Image
Flow Cytometry - Anti-ROCK1 antibody [EP786Y]; Overlay histogram showing HeLa cells stained with TA303688 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title