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Anti-ROCK1 Antibody EP786Y
Also for ROCK1 (NM_005406)
|A synthetic peptide corresponding to residues near C-terminus of human ROCK1 was used as an immunogen. This antibody recognizes both the cleaved C-terminus of ROCK-1 (30 kDa) and full length protein.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||IHC-Fr: 1:100; WB: 1:500; IHC-P: 1:50 - 1:100; ICC/IF: 1:250 - 1:500; IP: 1:50; FC: 1:1000
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens Rho-associated, coiled-coil containing protein kinase 1 (ROCK1)|
|This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq]. |
Signaling by GPCR
TGF Beta Signaling Pathway
Wnt Signaling Pathway
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Western blot - ROCK1 antibody [EP786Y]; All lanes : Anti-ROCK1 antibody [EP786Y] at 1/500 dilution.Lane 1 : Untreated HeLa cell lysate.Lane 2 : Camptothecin treated HeLa lysate.Predicted band size : 158 kDa.Observed band size : 30,158 kDa .
Immunohistochemistry (Paraffin-embedded sections) - ROCK1 antibody [EP786Y]; Ab45171 staining human ROCK1 in human thyroid gland carcinoma by immunohistochemistry using paraffin embedded tissue.
Immunocytochemistry/ Immunofluorescence - ROCK1 antibody [EP786Y]; Immunofluorescent staining of HeLa cells using ab45171.
Flow Cytometry - Anti-ROCK1 antibody [EP786Y]; Overlay histogram showing HeLa cells stained with ab45171 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.