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Anti-MAP2K5 Antibody EP648Y
Also for MAP2K5 (NM_002757)
|A synthetic peptide corresponding to residues within human MKK5 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:10,000; ICC: 1:100-250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens mitogen-activated protein kinase kinase 5 (MAP2K5), transcript variant 2|
|HsT17454; MAPKK5; MEK5; PRKMK5|
|The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been described. [provided by RefSeq]. |
EGFR1 Signaling Pathway
MAPK signaling pathway
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Western blot - MEK5 antibody [EP648Y]; Anti-MEK5 antibody [EP648Y] at 1/10000 dilution + HeLa cell lysate.Predicted band size : 49 kDa.Observed band size : 49 kDa.
Immunocytochemistry/ Immunofluorescence - MEK5 antibody [EP648Y]; Immunofluorescent staining of HeLa using ab45146.
Immunocytochemistry/ Immunofluorescence - MEK5 antibody [EP648Y]; ab45146 at 1/100 staining HeLa cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with goat serum prior to incubation with the antibody for 45 minutes. An Alexa Fluor® 488 conjugated goat polyclonal antibody was used as the secondary. This antibody detects MEK5 in HeLa cells as a cytoplasmic protein.