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Anti-ACTN2 Antibody EP2529Y
Also for ACTN2 (NM_001103)
|A synthetic peptide corresponding to residues on the N-terminus of human Alpha-Actinin 2 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IP: 1:40; IHC-P: 1:100 - 1:250; FC: 1:100
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for ICC/IF.
|Homo sapiens actinin, alpha 2 (ACTN2), transcript variant 1|
|Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. [provided by RefSeq]. |
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Western blot - Sarcomeric Alpha Actinin antibody [EP2529Y]; All lanes : Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] at 1/20000 dilution.Lane 1 : Human skeletal muscle lysate.Lane 2 : Rat heart lysate.Lane 3 : Human heart lysate.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 103 kDa.Observed band size : 103 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Sarcomeric Alpha Actinin antibody [EP2529Y]; ab68167 at 1/100 dilution staining Sarcomeric Alpha Actinin in human muscles by Immunohistochemistry, Paraffin-embedded tissue.