Home Antibody All anti-CAMK1G antibodies
Also for CAMK1G (NM_020439)
|Rabbits were immunized wth a KLH conjugated synthetic peptide selected from the C-terminal region of human CaMKIG.|
||WB: 1: 100~500; IHC: 1:50~100; ELISA: 1: 1,000;
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.|
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. (Protein A or G Sepharose)
|Homo sapiens calcium/calmodulin-dependent protein kinase IG (CAMK1G)|
|CLICK3; CLICKIII; dJ272L16.1; VWS1|
|Ca2+/calmodulin-dependent protein kinase I (CaMKI) constitutes a family of closely related isoforms (alpha, beta and gamma). CLICK-III/CaMKIgamma is a novel membrane-anchored neuronal Ca2+/calmodulin-dependent protein kinase. AMKIgamma is abundant in neurons, particularly in the amygdala and ventromedial hypothalamus. Like the other CaMKI isoforms, full activation of CLICK-III/CaMKIgamma requires both Ca(2+)/CaM and phosphorylation by CaMKK.|
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(LEFT)Western blot analysis of anti-CAMK 1G Pab in NCI-H460 cell lysate. CAMK 1G (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. (RIGHT)Western blot analysis of CAMK1G (arrow) using rabbit polyclonal CAMK1G Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CAMK1G gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.