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Home Antibody All anti-CDK4 antibodies

Anti-CDK4 Antibody EPR2513Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA301195
  • Rabbit Monoclonal Antibody against CDK4 (Clone EPR2513Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400021) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
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Also for CDK4 (NM_000075)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the N-terminus of human CDK4 was used as an immunogen.
Clone NameEPR2513Y IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB Suggested DilutionsWB: 1:500; FC: 1:60; IP: 1:30
Predicted MW Explanation 34 kDa
BufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens cyclin-dependent kinase 4 (CDK4)
Alternative NameCMM3; PSK-J3
Database LinkNP_000066
Entrez Gene 1019 Human
FunctionCyclin dependent kinases (CDK's) are kinases that interact with cyclins and regulate cell division. There is evidence that phosphorylation of the C-terminal region of Rb by Cdk4/6 initiates successive intramolecular interactions between the C-terminal region and the central pocket. The initial interaction displaces histone deacetylase from the pocket, blocking active transcriptional repression by Rb. These intramolecular interactions provide a molecular basis for sequential phosphorylation of Rb by Cdk4/6 and Cdk2. Cdk4/6 is activated early in G1, blocking active repression by Rb (1). In primary epidermal cells, it has been found that oncogenic Ras transiently decreases cyclin-dependent kinase (CDK) 4 expression in association with cell cycle arrest in G1 phase. CDK4 co-expression circumvents Ras growth suppression and induces invasive human neoplasia resembling squamous cell carcinoma. Tumorigenesis is dependent on CDK4 kinase function, with cyclin D1 required but not sufficient for this process. These data identify a new role for oncogenic Ras in CDK4 regulation and highlight the functional importance of CDK4 suppression in preventing uncontrolled growth (2).
Related PathwayProtein KinaseDruggable Genome Cell cyclep53 signaling pathwayTight junctionT cell receptor signaling pathwayPathways in cancerPancreatic cancerMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Cdk4 antibody [EPR2513Y]; All lanes : Anti-Cdk4 antibody [EPR2513Y] at 1/500 dilution.Lane 1 : HeLa cell lysate.Lane 2 : MCF7 cell lysate.Lane 3 : Romas cell lysate.Lane 4 : K452 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP conjugated Goat anti-rabbit at 1/2000 dilution.Predicted band size : 34 kDa.Observed band size : 34 kDa.

 

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