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Anti-CDK4 Antibody EPR2513Y
Also for CDK4 (NM_000075)
|A synthetic peptide corresponding to residues on the N-terminus of human CDK4 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:500; FC: 1:60; IP: 1:30
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Does not react with Mouse, Rat
|Homo sapiens cyclin-dependent kinase 4 (CDK4)|
|Cyclin dependent kinases (CDK's) are kinases that interact with cyclins and regulate cell division. There is evidence that phosphorylation of the C-terminal region of Rb by Cdk4/6 initiates successive intramolecular interactions between the C-terminal region and the central pocket. The initial interaction displaces histone deacetylase from the pocket, blocking active transcriptional repression by Rb. These intramolecular interactions provide a molecular basis for sequential phosphorylation of Rb by Cdk4/6 and Cdk2. Cdk4/6 is activated early in G1, blocking active repression by Rb (1). In primary epidermal cells, it has been found that oncogenic Ras transiently decreases cyclin-dependent kinase (CDK) 4 expression in association with cell cycle arrest in G1 phase. CDK4 co-expression circumvents Ras growth suppression and induces invasive human neoplasia resembling squamous cell carcinoma. Tumorigenesis is dependent on CDK4 kinase function, with cyclin D1 required but not sufficient for this process. These data identify a new role for oncogenic Ras in CDK4 regulation and highlight the functional importance of CDK4 suppression in preventing uncontrolled growth (2). |
TGF Beta Signaling Pathway
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Western blot - Cdk4 antibody [EPR2513Y]; All lanes : Anti-Cdk4 antibody [EPR2513Y] at 1/500 dilution.Lane 1 : HeLa cell lysate.Lane 2 : MCF7 cell lysate.Lane 3 : Romas cell lysate.Lane 4 : K452 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP conjugated Goat anti-rabbit at 1/2000 dilution.Predicted band size : 34 kDa.Observed band size : 34 kDa.