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Anti-AMPH Antibody EP2060Y
Also for AMPH (NM_001635)
|A synthetic peptide corresponding to residues within human Amphipysin was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||WB: 1:25,000 50,000; IHC: 1: 100 250; ICC: 1: 100 250; FC: 1: 15
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens amphiphysin (AMPH), transcript variant 1|
Entrez Gene 273 Human
Entrez Gene 218038 Mouse
Entrez Gene 60668 Rat
|Amphiphysin, a neuronal protein first identified in chicken synaptic membranes, is the autoantigen of Stiff-Man Syndrome (SMS) associated with breast cancer. Patient autoantibodies have a distinct pattern of reactivity with amphiphysin, and the dominant autoepitope is located in its C-terminal region, which contains an SH3 domain (1). Amphiphysin is expressed in many neurons, certain endocrine cell types, and spermatocytes (2). study suggests a link between amphiphysin I expression in cancer and amphiphysin I autoimmunity. The enhanced expression of amphiphysin I in some forms of cancer supports the hypothesis that amphiphysin family members may play a role in the biology of cancer cells (3). |
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Western blot - Amphiphysin antibody [EP2060Y]; Anti-Amphiphysin antibody [EP2060Y] at 1/100000 dilution + Human brain lysate at 10 ug.Secondary.Goat anti-Rabbit HRP labeled at 1/2000 dilution.Predicted band size : 76 kDa.Observed band size : 128 kDa .
Immunohistochemistry (Paraffin-embedded sections) - Amphiphysin antibody [EP2060Y]; Ab52646 (1/100) staining human Amphiphysin in human brain tissue by immunohistochemistry using paraffin embedded tissue.
Immunocytochemistry/ Immunofluorescence - Amphiphysin antibody [EP2060Y]; Immunofluorescent staining of SH-SYS5 cells using TA300974 (1/100).
Flow Cytometry-Anti-Amphiphysin antibody [EP2060Y](TA300974); Overlay histogram showing SH-SY5Y cells stained with TA300974 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.