Home Antibody All anti-MCM7 antibodies
Also for MCM7 (NM_005916)
|A synthetic peptide corresponding to residues near the N-terminus of human MCM7 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:5000; IP: 1:20
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified
|Does not react with Mouse
|Homo sapiens minichromosome maintenance complex component 7 (MCM7), transcript variant 1|
|CDC47; MCM2; P1.1-MCM3; P1CDC47; P85MCM; PNAS146|
|The minichromosome maintenance (MCM7) helicase complex functions to initiate and elongate replication forks. Cell cycle checkpoint signaling pathways regulate DNA replication to maintain genomic stability (1). MCM7 is a putative human homologue of yeast CDC47 and a member of the MCM protein family, which has been implicated in the regulatory machinery causing DNA to replicate only once in the S phase. Findings indicate that MCM7 protein together with other MCM proteins participates in the regulation of mammalian DNA replication (2). In HeLa cells, depletion of MCM7 with small-interfering RNA suppressed ultraviolet (UV) light- or aphidicolin-induced hChk1 phosphorylation, and abolished UV-induced S-phase checkpoint activation. These results demonstrate that MCM7 plays a direct role in the transmission of DNA damage signals from active replication forks to the S-phase checkpoint machinery in human cells (3). |
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Western blot - MCM7 antibody; Anti-MCM7 antibody at 1/5000 dilution + HeLa cell lysate at 10 µg.Secondary.Goat anti-Rabbit HRP labeled. at 1/2000 dilution.Predicted band size : 81 kDa.Observed band size : 81 kDa.