OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-SNCA antibodies

Anti-SNCA Antibody EP1646Y

div

Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA300914 Rabbit Monoclonal Antibody against alpha-synuclein (SNCA) (Clone EP1646Y) 100ul $325 In Stock
LC400127 SNCA HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
Add to Shopping Cart
spacer
WB(1)
IF(2)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the N-terminus of human Alpha-synuclein was used as an immunogen.
Clone NameEP1646Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF, FC Suggested DilutionsFC: 1:1000; WB: 1:50000; ICC/IF: 1:50 - 1:100
Predicted MW Explanation 14 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IHC-Fr,IHC-P or IP.

Reference Data

Target NameHomo sapiens synuclein, alpha (non A4 component of amyloid precursor) (SNCA), transcript variant 1
Alternative NameNACP; PARK1; PARK4; PD1
Database LinkNP_000336
Entrez Gene 6622 Human
Entrez Gene 20617 Mouse
Entrez Gene 29219 Rat
FunctionAlpha-synuclein is expressed predominantly in brain, where it is concentrated in presynaptic nerve terminals (1). The deposition of the abundant presynaptic brain protein alpha-synuclein as fibrillary aggregates in neurons or glial cells is a hallmark lesion in a subset of neurodegenerative disorders. These disorders include Parkinson's disease (PD), dementia with Lewy bodies (DLB) and multiple system atrophy, collectively referred to as synucleinopathies. Phosphorylation of alpha-synuclein at Ser 129 promoted fibril formation in vitro and these results highlight the importance of phosphorylation of filamentous proteins in the pathogenesis of neurodegenerative disorders (2). Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin. It is hypothesized that parkin and alpha-synculein interact functionally, namely, that parkin ubiquitinates alpha-synuclein normally and that this process is altered in autosomal recessive PD (3).
Related PathwayDruggable Genome Alzheimer's diseaseParkinson's disease

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - alpha Synuclein antibody [EP1646Y]; Anti-alpha Synuclein antibody [EP1646Y] at 1/50000 dilution + Fetal brain lysate at 10 ug.Secondary.Goat anti-Rabbit HRP labeled. at 1/2000 dilution.Predicted band size : 14 kDa.Observed band size : 18 kDa .
spacer
Immunocytochemistry/ Immunofluorescence - alpha Synuclein antibody [EP1646Y]; Immunofluorescent staining of PC12 cells using TA300914 (1:50).
spacer
Immunocytochemistry/ Immunofluorescence - Anti-alpha Synuclein antibody [EP1646Y]; Immunofluorescence analysis of rat primary hippocampal neurons, staining alpha Synuclein with TA300914.Cells were fixed, permeabilized, and blocked with 10% donkey serum at room temperature. Cells were incubated with primary antibody (1/1000) at 4°C for 24 hours. A Cy2-conjugated donkey anti-rabbit IgG was used as the secondary antibody.
spacer
Flow Cytometry - Anti-alpha Synuclein antibody; Overlay histogram showing SH-SY5Y cells stained with TA300914 (red line). The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1:2000. Isotype control antibody (black line) was rabbit IgG (monoclonal) used under the same conditions. Unlabelled sample (blue line) was also used as a control.
spacer

 

spacer
Inc 5000 Healthcare Company
All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title

Notice to Non-US Customers

ExclaimationWeb price and delivery time are for direct sales only.

Non-US customers are encouraged to contact our dedicated distributor network for quotes and streamlined ordering/delivery support.

Acknowledge