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Home Antibody All anti-SNCA antibodies

Anti-SNCA Antibody EP1646Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300914
  • Rabbit Monoclonal Antibody against alpha-synuclein (SNCA) (Clone EP1646Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400127) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IF(2)
FC(1)
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Also for SNCA (NM_000345)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the N-terminus of human Alpha-synuclein was used as an immunogen.
Clone NameEP1646Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF, FC Suggested DilutionsFC: 1:1000; WB: 1:50000; ICC/IF: 1:50 - 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IHC-Fr,IHC-P or IP.

Reference Data

Target NameHomo sapiens synuclein, alpha (non A4 component of amyloid precursor) (SNCA), transcript variant 1
Alternative NameNACP; PARK1; PARK4; PD1
Database LinkNP_000336
FunctionAlpha-synuclein is expressed predominantly in brain, where it is concentrated in presynaptic nerve terminals (1). The deposition of the abundant presynaptic brain protein alpha-synuclein as fibrillary aggregates in neurons or glial cells is a hallmark lesion in a subset of neurodegenerative disorders. These disorders include Parkinson's disease (PD), dementia with Lewy bodies (DLB) and multiple system atrophy, collectively referred to as synucleinopathies. Phosphorylation of alpha-synuclein at Ser 129 promoted fibril formation in vitro and these results highlight the importance of phosphorylation of filamentous proteins in the pathogenesis of neurodegenerative disorders (2). Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin. It is hypothesized that parkin and alpha-synculein interact functionally, namely, that parkin ubiquitinates alpha-synuclein normally and that this process is altered in autosomal recessive PD (3).
Related Pathway
EGFR1 Signaling Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - alpha Synuclein antibody [EP1646Y]; Anti-alpha Synuclein antibody [EP1646Y] at 1/50000 dilution + Fetal brain lysate at 10 ug.Secondary.Goat anti-Rabbit HRP labeled. at 1/2000 dilution.Predicted band size : 14 kDa.Observed band size : 18 kDa .
IF Image
Immunocytochemistry/ Immunofluorescence - alpha Synuclein antibody [EP1646Y]; Immunofluorescent staining of PC12 cells using TA300914 (1:50).
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-alpha Synuclein antibody [EP1646Y]; Immunofluorescence analysis of rat primary hippocampal neurons, staining alpha Synuclein with TA300914.Cells were fixed, permeabilized, and blocked with 10% donkey serum at room temperature. Cells were incubated with primary antibody (1/1000) at 4°C for 24 hours. A Cy2-conjugated donkey anti-rabbit IgG was used as the secondary antibody.
FC Image
Flow Cytometry - Anti-alpha Synuclein antibody [EP1646Y]; Overlay histogram showing SH-SY5Y cells stained with TA300914 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

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