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Anti-SUFU Antibody EP1207Y
Also for SUFU (NM_016169)
|A synthetic peptide corresponding to peptides on human Su(Fu) was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:500; IP: 1:50; ICC: 1:100 - 1:250; FC: 1:100; IHC-P: Use at an assay dependent dilution; ICC/IF: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens suppressor of fused homolog (Drosophila) (SUFU), transcript variant 1|
|PRO1280; SUFUH; SUFUXL|
|The Hedgehog (Hh) signaling pathway has conserved roles in development of species ranging from Drosophila to humans. Human supressor of fused HSu(Fu) acts as a negative regulator in the hedgehog signaling (SHH) pathway by down-regulating GLI1-mediated transactivation of target genes (1). HSu(fu) was found to repress activity of the zinc-finger transcription factor Gli, which mediates Hedgehog signaling in vertebrates, and to physically interact with Gli, Gli2 and Gli3 (2). The sonic hedgehog signaling pathway directs the embryonic development of diverse organisms and is disrupted in a variety of malignancies. Several of mutations encode truncated proteins that are unable to export the GLI transcription factor from nucleus to cytoplasm, resulting in the activation of SHH signaling. SUFU is a newly identified tumor-suppressor gene that predisposes individuals to medulloblastoma by modulating the SHH signaling pathway through a newly identified mechanism (3). |
Hedgehog signaling pathway
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Western blot - Suppressor of Fused antibody [EP1207Y]; Anti-Suppressor of Fused antibody [EP1207Y] at 1/200 dilution + LnCaP cell lysate at 10 µg.Secondary.Goat anti rabbit HRP antibody at 1/2000 dilution.Predicted band size : 54 kDa.Observed band size : 54 kDa.
Immunohistochemistry (Paraffin-embedded sections) - Suppressor of Fused antibody [EP1207Y]; Ab52913 at 1/100 dilution staining human fetal kidney tissue; paraffin embedded.
Flow Cytometry-Suppressor of Fused antibody [EP1207Y](ab52913); Overlay histogram showing HEK293 cells stained with ab52913 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) ( 1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.