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Anti-PTK2 Antibody EP695Y
Also for PTK2 (NM_153831)
|A synthetic peptide corresponding to residues in human FAK was used as immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||FC: 1:100; ICC/IF: Use at an assay dependent concentration; IHC-P: Use at an assay dependent dilution; ICC: 1:250 - 1:500; WB: 1:1000; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|IgG fraction (Protein A or G Sepharose)
|Homo sapiens protein tyrosine kinase 2 (PTK2), transcript variant 1|
|FADK; FAK; FAK1; FRNK; p125FAK; pp125FAK; PPP1R71|
|Focal adhesion kinase (FAK) is a non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis (1). FAK is composed of a central catalytic domain flanked by large N- and C-terminal regions. FAK is activated by phosphorylation at tyrosine 397 in response to integrin clustering which can be induced by cell adhesion or antibody cross-linking or via G-protein-coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid (2-3). FAK expression is upregulated in various tumors (4). Increased FAK expression has been correlated with the enhanced motility and invasiveness of human tumor cells, as well as with promoting increased cell proliferation (5).|
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Western blot - FAK antibody [EP695Y]; Anti-FAK antibody [EP695Y] at 1/1000 dilution + Hela cell lysate.Predicted band size : 119 kDa.Observed band size : 119 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FAK antibody [EP695Y]; Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using ab40794.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FAK antibody [EP695Y]; The image shows FAK antibody in human spleen tissue. Clear cytoplasmic positivity in a subset of germinal centre cells. The there is intense positivity of the serum in the blood vessels. Endogenous peroxidases was blocked using 2% H2O2, for 15 minutes.
Immunofluorescence - FAK antibody [EP695Y]; Immunofluorescent staining of HeLa cells using ab40794.
Flow Cytometry-FAK antibody [EP695Y](ab40794); Overlay histogram showing HeLa cells stained with ab40794 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.