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Home Antibody All anti-PTK2 antibodies

Anti-PTK2 Antibody EP695Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300620
  • Rabbit Monoclonal Antibody against PTK2 (Clone EP695Y)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC403521) , 20ug
100ul 325 3-7 Days
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WB(1)
IHC(2)
IF(1)
FC(1)
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Also for PTK2 (NM_153831)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human FAK was used as immunogen.
Clone NameEP695Y IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsFC: 1:100; ICC/IF: Use at an assay dependent concentration; IHC-P: Use at an assay dependent dilution; ICC: 1:250 - 1:500; WB: 1:1000; IP: 1:50
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification IgG fraction

Reference Data

Target NameHomo sapiens protein tyrosine kinase 2 (PTK2), transcript variant 1
Alternative NameFADK; FAK; FAK1; FRNK; p125FAK; pp125FAK; PPP1R71
Database LinkNP_722560
FunctionFocal adhesion kinase (FAK) is a non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis (1). FAK is composed of a central catalytic domain flanked by large N- and C-terminal regions. FAK is activated by phosphorylation at tyrosine 397 in response to integrin clustering which can be induced by cell adhesion or antibody cross-linking or via G-protein-coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid (2-3). FAK expression is upregulated in various tumors (4). Increased FAK expression has been correlated with the enhanced motility and invasiveness of human tumor cells, as well as with promoting increased cell proliferation (5).
Related Pathway
Focal Adhesion
Hemostasis

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WB Image
Western blot - FAK antibody [EP695Y]; Anti-FAK antibody [EP695Y] at 1/1000 dilution + Hela cell lysate.Predicted band size : 119 kDa.Observed band size : 119 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FAK antibody [EP695Y]; Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using TA300620.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FAK antibody [EP695Y]; The image shows FAK antibody in human spleen tissue. Clear cytoplasmic positivity in a subset of germinal centre cells. The there is intense positivity of the serum in the blood vessels. Endogenous peroxidases was blocked using 2% H2O2, for 15 minutes.
IF Image
Immunofluorescence - FAK antibody [EP695Y]; Immunofluorescent staining of HeLa cells using TA300620.
FC Image
Flow Cytometry-FAK antibody [EP695Y](TA300620); Overlay histogram showing HeLa cells stained with TA300620 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.

 

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