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Anti-BAK1 Antibody Y164
Also for BAK1 (NM_001188)
|A synthetic peptide corresponding to residues near the N-term of human Bak was used as immunogen. The antibody does not cross-react with other Bcl-2 members.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:1000 - 1:5000; IHC-P: Use at an assay dependent concentration; ICC: 1:250 - 1:500; FC: 1:10; IP: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|IgG fraction (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens BCL2-antagonist/killer 1 (BAK1)|
|BAK; BAK-LIKE; BCL2L7; CDN1|
|Bak a pro-apoptotic analog to Bax, is a member of the Bcl-2 family of proteins. During mitochondria-regulated programmed cell death, Bak promotes apoptosis by binding Bcl-2 and inhibiting its anti-apoptotic function (1). Upon apoptotic stimulation, Bak translocates from the cytosol to the mitochondrial membrane (2). Pro-apoptotic cascade activates BID, which oligomerizes Bak into pores that result in the release of cytochrome c (2, 3). In healthy cells, Bak associate with Bcl-xL and Mcl-1 but not with Bcl-2, Bcl-w or A1 and only when freed of both Bak will induce apoptosis. |
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Western blot - Bak antibody [Y164]; Anti-Bak antibody [Y164] at 1/5000 dilution + HeLa cell lysate.Predicted band size : 23 kDa.Observed band size : 23 kDa.
Immunohistochemistry (Paraffin-embedded sections) - Bak antibody [Y164]; Immunohistochemical analysis of Bak expression in paraffin embedded human stomac carcinoma, using 1/250 ab32371.
Immunocytochemistry/ Immunofluorescence-Bak antibody [Y164](ab32371); ICC/IF image of ab32371 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.