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Home Antibody All anti-BAK1 antibodies

Anti-BAK1 Antibody Y164

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300476
  • Rabbit Monoclonal Antibody against BAK1 (Clone Y164)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC400476) , 20ug
100ul 325 3-7 Days
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WB(1)
IHC(1)
IF(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-term of human Bak was used as immunogen. The antibody does not cross-react with other Bcl-2 members.
Clone NameY164 IsotypeIgG
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:1000 - 1:5000; IHC-P: Use at an assay dependent concentration; ICC: 1:250 - 1:500; FC: 1:10; IP: 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification IgG fraction
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens BCL2-antagonist/killer 1 (BAK1)
Alternative NameBAK; BAK-LIKE; BCL2L7; CDN1
Database LinkNP_001179
FunctionBak a pro-apoptotic analog to Bax, is a member of the Bcl-2 family of proteins. During mitochondria-regulated programmed cell death, Bak promotes apoptosis by binding Bcl-2 and inhibiting its anti-apoptotic function (1). Upon apoptotic stimulation, Bak translocates from the cytosol to the mitochondrial membrane (2). Pro-apoptotic cascade activates BID, which oligomerizes Bak into pores that result in the release of cytochrome c (2, 3). In healthy cells, Bak associate with Bcl-xL and Mcl-1 but not with Bcl-2, Bcl-w or A1 and only when freed of both Bak will induce apoptosis.
Related Pathway
Apoptosis

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WB Image
Western blot - Bak antibody [Y164]; Anti-Bak antibody [Y164] at 1/5000 dilution + HeLa cell lysate.Predicted band size : 23 kDa.Observed band size : 23 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Bak antibody [Y164]; Immunohistochemical analysis of Bak expression in paraffin embedded human stomac carcinoma, using 1/250 TA300476.
IF Image
Immunocytochemistry/ Immunofluorescence-Bak antibody [Y164](TA300476); ICC/IF image of TA300476 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

 

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