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Anti-BAD Antibody Y208


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA300475 Rabbit Monoclonal Antibody against BAD (Clone Y208) 100ul $325 In Stock
LC403214 BAD HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the N-term of human Bad was used as immunogen. The antibody does not cross-react with other Bcl-2 members.
Clone NameY208 IsotypeIgG
Species ReactivityMouse, Rat, Dog, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:2000 - 1:5000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:250 - 1:500; FC: 1:20 - 1:50; IP: 1:100
Predicted MW Explanation 18 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Protein A purified

Reference Data

Target NameBCL2 associated agonist of cell death
Alternative NameBBC2|BCL2L8
Database LinkNP_116784
Entrez Gene 572 Human
Entrez Gene 12015 Mouse
Entrez Gene 64639 Rat
Entrez Gene 483763 Dog
FunctionBad is a pro-apoptotic member of the Bcl-2 family. Bad will bind preferentially Bcl-xL over Bcl-2 through its partial (9-16 a.a) BH3 domain. Additionally, Bad can reverse the death repressor activity of Bcl-xL but not of Bcl-2. Upon dephosphorylation, activated Bad translocates to the mitochondria and displace Bcl-2 from tBid sequestration to initiate mitochondrial dysfunction. The switching on/off of its phosphorylation by growth/survival factors regulates Bad activity.
Related PathwayDruggable Genome ErbB signaling pathwayApoptosisVEGF signaling pathwayFocal adhesionNeurotrophin signaling pathwayInsulin signaling pathwayMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Bad antibody [Y208]; Anti-Bad [Y208] antibody at 1/5000 dilution + Hela cell lysate.Predicted band size : 18 kDa.Observed band size : 23 kDa .

IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Bad antibody [Y208]; Immunohistochemical staining of paraffin-embedded human lymphoma using TA300475 at 1/100 dilution.

IF Image
ICC/IF image of TA300475 stained MCF7 cells. The cells were incubated with the antibody overnight at 4.. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at 1:1000 for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at 1:200 for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.

FC Image
Flow Cytometry-Bad antibody(TA300475); Overlay histogram showing MCF-7 cells stained with TA300475 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit monoclonal IgG (1ug/1x10^6 cells) used under the same conditions. This antibody gave a decreased signal in MCF-7 cells under the same conditions.


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