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Anti-BIRC3 Antibody E40
Also for BIRC3 (NM_001165)
|A synthetic peptide corresponding to residues, before the breakpoint for translocation to form BIRC3-MALT1, of Human BIP3 was used as immunogen. The antibody does not cross-react with other IAP family members.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IP
||WB: 1:1000; IHC-P: Use at an assay dependent dilution; ICC: 1:100; IP: Use a concentration of 5 ug/ml
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens baculoviral IAP repeat containing 3 (BIRC3), transcript variant 1|
|AIP1; API2; c-IAP2; CIAP2; HAIP1; HIAP1; MALT2; MIHC; RNF49|
|cIAP-2, also known as HIAP-1, is a member of the inhibitor of apoptosis (IAP) family of proteins. These proteins contain a BIR (baculovirus IAP repeat) domain near the amino-terminus (1). cIAP-2 is localized to the cytoplasm. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form a heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2). This complex is thought to play a role in inhibition of apoptosis (2,3). cIAP-2 inhibits cytochrome c-induced activation of caspase-9, thereby preventing initiation of the caspase cascade and apoptosis (4).|
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Western blot - cIAP2 antibody [E40]; Anti-cIAP2 antibody [E40] at 1/1000 dilution + Daudi cell lysate.Predicted band size : 72 kDa.Observed band size : 72 kDa.
Immunohistochemistry (Paraffin-embedded sections) - cIAP2 antibody [E40]; ab32059 at a 1:50 dilution staining cIAP2 in human normal spleen, using Immunohistochemistry, Paraffin Embedded Tissue.
Immunoprecipitation - Anti-cIAP2 antibody [E40]; cIAP2 was immunoprecipitated using 0.5mg Daudi whole cell extract, 5µg of Rabbit polyclonal to cIAP2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). .The antibody was incubated under agitation with Protein G beads for 10min, Daudi whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32059.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) .Band: 72kDa; cIAP2