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Home Antibody All anti-PXN antibodies

Anti-PXN Antibody E228

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300327
  • Rabbit Monoclonal Antibody against PXN (Clone E228)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC401010) , 20ug
100ul 325 3-7 Days
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WB(1)
IHC(1)
IF(2)
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Also for PXN (NM_002859)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Tyr31 of human Paxillin was used as immunogen. The antibody only detects Paxillin phosphorylated on Tyrosine 31.
Clone NameE228 IsotypeIgG
Species ReactivityHuman, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:5000; IHC: 1: 100-1:250; ICC: 1:250; IP: 1: 100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification IgG fraction

Reference Data

Target NameHomo sapiens paxillin (PXN), transcript variant 1
Alternative NameFLJ16691
Database LinkNP_002850
FunctionPaxillin is a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion) (1). Paxillin plays a significant role in integrin signaling and the tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by focal adhesion kinase (FAK) at Tyr118 (3). It has been found that phosphorylation of paxillin at both Tyr118 and Tyr31 is essential for MM1 cancer cell migration (4).
Related Pathway
EGFR1 Signaling Pathway
Focal Adhesion

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WB Image
Western blot - Paxillin (phospho Y31) antibody [E228]; All lanes : Anti-Paxillin (phospho Y31) antibody [E228] at 1/10000 dilution.Lane 1 : untreated NIH 3T3 cell lysate.Lane 2 : PDGF treated NIH 3T3 cell lysate.Predicted band size : 64 kDa.Observed band size : 64 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Paxillin (phospho Y31) antibody [E228]; Immunohistochemical analysis of Paxillin (phospho Y31) expression in paraffin-embedded human colon carcinoma using 1/100 TA300327.
IF Image
Immunocytochemistry/ Immunofluorescence - Paxillin (phospho Y31) antibody [E228]; Immunofluorescent analysis of Paxillin (phospho Y31) expression in HeLa cell culture, using 1/250 TA300327.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Paxillin (phospho Y31) antibody [E228]; Immunofluorescence analysis of HeLa cells, staining Paxillin (phospho Y31) with TA300327. Cells on the right were treated with MGAT1 shRNA. Cells were fixed with 2% paraformaldehyde, permeabilized using 0.2% Triton-X-100 and blocked by 5% BSA for 1 hour. Cells were incubated with primary antibody (1/400) overnight at 4°C. A FITC-conjugated donkey anti-rabbit IgG (1/500) was used as the secondary antibody.

 

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