RNA (clinical derived) FAQs
1. Do you offer matched normal and tumor RNA from the same patient?
We have some matching samples and the easiest way to find them is to look for case IDs that are hyperlinked. By clicking on these case ID numbers, you will be able to view all tissues from the same donor.
Or one can search the tissue type of interest and select all (both tumor and normals) to download onto the spreadsheet. Once you have the spreadsheet sort it by Case ID # (CIxxxxxxxxxxxxxx). All samples with the same case ID number come from the same patient.
2. How was the RNA purified?
RNA was purified using a Trizol-based method.
3. Does the RNA contain small RNAs like miRNA and siRNA?
The RNA purification method employed was not designed for the preservation of small RNA species and although the samples may contain some small RNAs, these may not truly represent the small RNA profile of the starting tissue sample.
4. Was the RNA treated with DNAse?
Yes, the RNA was treated with DNAse. A PCR amplification against on a known genomic amplicon was tested against positive controls to ensure the absence of genomic DNA contamination.
5. Some of my samples have additional information at the bottom of the page under Diagnosis. What does this mean?
For some samples, our database contains additional information on the samples such as Her2/ERBB2 status (~ means = ). We include this information when it is available. If a sample does not show anything in this bottom row of the table, then no additional testing was performed on this sample.
6. Are the RNA samples pooled?
No. Every RNA sample is obtained from a single tissue block. All of the clinical information about that tissue is presented on the web and upon further request the pictures of the H&E stains from that block can be obtained through firstname.lastname@example.org.
7. What is the quality of the RNA provided?
All RNA samples are evaluated with an Agilent Bioanalyzer and minimum passing RIN scores must be obtained. Quantitation is assessed by OD260 on a Nanodrop spectrophotometer.
8. How are the RNA samples stored and shipped?
While there should not be any active RNAse activity in the samples, every vial is stored at -80C and is shipped on dry ice. That being said, storage at your facility at -20C should be sufficient for at least 6 months.
9. What is the source of your human tissue blocks?
OriGene, through its acquisition of Cytomyx (previously Ardais), maintains well over 130,000 human tissue blocks that were obtained through strict IRB protocols and with full, documented patient consent all from accredited US-based medical institutions. OriGene tissue bank is the repository of choice for several pharmaceutical companies because of its quality and reputation.