MolBio Tools for COVID-19 Research (SARS-CoV-2 infection)

COVID19 is caused by a newly identified coronavirus named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Early studies have shown SARS-CoV-2 attaches to the human cells through an interaction between viral “Spike” protein and human ACE2 receptors; after binding to the cell surface, the “Spike” protein gets cleaved by human TMPRSS2 protease to facilitate the entry of virus.

To help researchers further dissect the molecular mechanism of this new virus, OriGene has developed various MolBio products covering all the SARS-CoV-2 virus genes and many human genes that play important roles in the pathogenesis of the virus.

Gene centric MolBio tools for COVID-19 research including clones, CRISPR kits, qPCR reagents, RNAi for SARS-CoV-2 & human genes

Gene centric MolBio tools for COVID-19 research
Clones | RNAi | CRISPR kits | qPCR

  • Receptor & Activator


    • Angiotensin-converting enzyme 2 (ACE2) is a membrane protein that has been found to play an important role in the infection of SARS-CoV-2 virus. The protein contains 805 amino acids with a N-terminal signal peptide, a transmembrane domain and a cytoplasmic domain. It is expressed in many tissues of human body, which may address the reason of non-respiratory symptoms seen in some COVID-19 patients . To many researchers, ACE2 has become a hot target due to the recent outbreak of COVID-19.

      • Human ACE2 MolBio Toolset
        Application Catalog# Product Description
        Overexpress ACE2
        proteinin Mammalian
        cells         		SC124099 Untagged cDNA clone encodes native ACE2 protein.
        RC208442 ORF clone encodes C-terminal Myc-DDK tagged ACE2 protein
        RG208442 ORF clone encodes C-terminal tGFP tagged ACE2 protein
        RC208442L1 Lenti ORF clone with C-terminal Myc-DDK tag
        RC208442L2 Lenti ORF clone with C-terminal mGFP tag
        RC208442L3 Lenti ORF clone with Myc-DDK tag and puro selection marker
        RC208442L4 Lenti ORF clone with mGFP tag and puro selection marker
        RC208442L1V Lentiviral particle made from RC208442L1
        RC208442L2V Lentiviral particle made from RC208442L2
        RC208442L3V Lentiviral particle made from RC208442L3
        RC208442L4V Lentiviral particle made from RC208442L4
        Knockdown
        ACE2 expression at
        mRNA level         		TR306881 Retroviral shRNA DNA including 4 shRNA & 1 scrambled control
        TL306881 Lentivrial shRNA DNA including 4 shRNA & 1 scrambled control
        TL306881V Lentiviral shRNA particles made from TL306881
        SR311790 siRNA to knockdown the ACE2 expression
        Genome Editing KN408442 CRISPR gene knockout kit for human ACE2
        GA111814 CRISPR gene activation kit for human ACE2
        qPCR Quantification HP214223 qPCR primer pair for quantification of human ACE2 cDNA level
        HK212668 qPCR template standard for the calculation of exact copy number
      • Mouse ACE2 MolBio Toolset
        Application Catalog# Product Description
        Overexpress ACE2
        proteinin Mammalian
        cells         		MC221763 Untagged cDNA clone encodes native ACE2 protein.
        MR210755 ORF clone encodes C-terminal Myc-DDK tagged ACE2 protein
        MG210755 ORF clone encodes C-terminal tGFP tagged ACE2 protein
        MR210755L3 Lenti ORF clone with Myc-DDK tag and puro selection marker
        MR210755L4 Lenti ORF clone with mGFP tag and puro selection marker
        MR210755L3V Lentiviral particle made from MR210755L3
        MR210755L4V Lentiviral particle made from MR210755L4
        Knockdown
        ACE2 expression at
        mRNA level         		TR504379 Retroviral shRNA DNA including 4 shRNA & 1 scrambled control
        TL504379 Lentivrial shRNA DNA including 4 shRNA & 1 scrambled control
        TL504379V Lentiviral shRNA particles made from TL504379
        SR420297 siRNA to knockdown the ACE2 expression
        Genome Editing KN500711 CRISPR gene knockout kit for mouse ACE2
        GA208393 CRISPR gene activation kit for mouse ACE2
        qPCR Quantification MP220690 qPCR primer pair for quantification of mouse ACE2 cDNA level
      • Rat ACE2 MolBio Toolset
        Application Catalog# Product Description
        Overexpress ACE2
        proteinin Mammalian
        cells         		RN214151 Untagged cDNA clone encodes native ACE2 protein.
        RR214151 ORF clone encodes C-terminal Myc-DDK tagged ACE2 protein
        RR214151L3 Lenti ORF clone with Myc-DDK tag and puro selection marker
        RR214151L4 Lenti ORF clone with mGFP tag and puro selection marker
        RR214151L3V Lentiviral particle made from RR214151L3
        RR214151L4V Lentiviral particle made from RR214151L4
        Knockdown
        ACE2 expression at
        mRNA level         		TR701518 Retroviral shRNA DNA including 4 shRNA & 1 scrambled control
        TL701518 Lentivrial shRNA DNA including 4 shRNA & 1 scrambled control
        TL701518V Lentiviral shRNA particles made from TL701518
        SR513861 siRNA to knockdown the ACE2 expression

    • Many coronaviruses including HCoV-229E, MERS-CoV, SARS-CoV and SARS-CoV-2 (COVID-19 virus) are found to use the same protease TMPRSS2 for the entry of the virus. TMPRSS2 is a serine protease that can cleave and activate the S-protein of the virus, which leads to the entry of the virus. Two spliced variants encoding different isoforms have been found for this gene.

      • Human TMPRSS2 Variant 1 MolBio Toolset
        Application Catalog# Product Description
        Overexpress TMPRSS2
        isoform 1 protein (long
        isoform) in Mammalian
        cells         		SC327067 Untagged cDNA clone encodes native TMPRSS2 isoform 1 protein
        RC228432 ORF clone encodes C-terminal Myc-DDK tagged TMPRSS2 isoform 1 protein
        RG228432 ORF clone encodes C-terminal tGFP tagged TMPRSS2 isoform 1 protein
        RC228432L1 Lenti ORF clone with C-terminal Myc-DDK tag
        RC228432L2 Lenti ORF clone with C-terminal mGFP tag
        RC228432L3 Lenti ORF clone with Myc-DDK tag and puro selection marker
        RC228432L4 Lenti ORF clone with mGFP tag and puro selection marker
        RC228432L1V Lentiviral particle made from RC228432L1
        RC228432L2V Lentiviral particle made from RC228432L2
        RC228432L3V Lentiviral particle made from RC228432L3
        RC228432L4V Lentiviral particle made from RC228432L4
        3’ UTR Clone
        miRNA regulation         		SC215536 3’UTR clone to study the miRNA regulation of TMPRSS2 expression
        Knockdown
        TMPRSS2 expression
        at mRNA level (work for both isoforms)         		TR308745 Retroviral shRNA DNA including 4 shRNA & 1 scrambled control
        TL308745 Lentivrial shRNA DNA including 4 shRNA & 1 scrambled control
        TL308745V Lentiviral shRNA particles made from TL308745
        SR322062 siRNA to knockdown the TMPRSS2 expression
        Genome Editing KN408677 CRISPR gene knockout kit for human TMPRSS2
        GA104914 CRISPR gene activation kit for human TMPRSS2
        qPCR Quantification HP208758 qPCR primer pair for quantification of human TMPRSS2 cDNA level
        HK207474 qPCR template standard for the calculation of exact copy number
      • Human TMPRSS2 Variant 2 MolBio Toolset
        Application Catalog# Product Description
        Overexpress TMPRSS2
        isoform 2 protein (short
        isoform) in Mammalian
        cells         		SC323858 Untagged cDNA clone encodes native TMPRSS2 isoform 2 protein
        RC208677 ORF clone encodes C-terminal Myc-DDK tagged TMPRSS2 isoform 2 protein
        RG208677 ORF clone encodes C-terminal tGFP tagged TMPRSS2 isoform 2 protein
        RC208677L1 Lenti ORF clone with C-terminal Myc-DDK tag
        RC208677L2 Lenti ORF clone with C-terminal mGFP tag
        RC208677L3 Lenti ORF clone with Myc-DDK tag and puro selection marker
        RC208677L4 Lenti ORF clone with mGFP tag and puro selection marker
        RC208677L1V Lentiviral particle made from RC208677L1
        RC208677L2V Lentiviral particle made from RC208677L2
        RC208677L3V Lentiviral particle made from RC208677L3
        RC208677L4V Lentiviral particle made from RC208677L4
        3’ UTR Clone
        miRNA regulation         		SC215537 3’UTR clone to study the miRNA regulation of TMPRSS2 expression
        Knockdown
        TMPRSS2 expression
        at mRNA level (work for both isoforms)         		TR308745 Retroviral shRNA DNA including 4 shRNA & 1 scrambled control
        TL308745 Lentivrial shRNA DNA including 4 shRNA & 1 scrambled control
        TL308745V Lentiviral shRNA particles made from TL308745
        SR322062 siRNA to knockdown the TMPRSS2 expression
        Genome Editing KN408677 CRISPR gene knockout kit for human TMPRSS2
        GA104914 CRISPR gene activation kit for human TMPRSS2
        qPCR Quantification HP208758 qPCR primer pair for quantification of human TMPRSS2 cDNA level
        HK207474 qPCR template standard for the calculation of exact copy number
      • Mouse TMPRSS2 MolBio Toolset
        Application Catalog# Product Description
        Overexpress TMPRSS2
        protein in Mammalian cells         		MC204660 Untagged cDNA clone encodes native TMPRSS2 protein.
        MR207852 ORF clone encodes C-terminal Myc-DDK tagged TMPRSS2 protein
        MG207852 ORF clone encodes C-terminal tGFP tagged TMPRSS2 protein
        MR207852L3 Lenti ORF clone with Myc-DDK tag and puro selection marker
        MR207852L4 Lenti ORF clone with mGFP tag and puro selection marker
        MR207852L3V Lentiviral particle made from MR207852L3
        MR207852L4V Lentiviral particle made from MR207852L4
        Knockdown
        TMPRSS2 expression at
        mRNA level         		TR502817 Retroviral shRNA DNA including 4 shRNA & 1 scrambled control
        TL502817 Lentivrial shRNA DNA including 4 shRNA & 1 scrambled control
        TL502817V Lentiviral shRNA particles made from TL502817
        SR415788 siRNA to knockdown the TMPRSS2 expression
        Genome Editing KN517940 CRISPR gene knockout kit for mouse TMPRSS2
        qPCR Quantification MP217322 qPCR primer pair for quantification of mouse TMPRSS2 cDNA level
        MK203882 qPCR template standard for the calculation of exact copy number
      • Rat TMPRSS2 MolBio Toolset
        Application Catalog# Product Description
        Overexpress TMPRSS2
        protein in Mammalian cells         		RN214298 Untagged cDNA clone encodes native TMPRSS2 protein.
        RR214298 ORF clone encodes C-terminal Myc-DDK tagged TMPRSS2 protein
        RR214298L3 Lenti ORF clone with Myc-DDK tag and puro selection marker
        RR214298L4 Lenti ORF clone with mGFP tag and puro selection marker
        RR214298L3V Lentiviral particle made from RR214298L3
        RR214298L4V Lentiviral particle made from RR214298L4
        Knockdown
        TMPRSS2 expression at
        mRNA level         		TR712521 Retroviral shRNA DNA including 4 shRNA & 1 scrambled control
        TL712521 Lentivrial shRNA DNA including 4 shRNA & 1 scrambled control
        TL712521V Lentiviral shRNA particles made from TL712521

  • SARS-CoV-2 Virus Genes

    • qPCR is the gold standard for gene expression analysis. It is a critical part of OriGene’s “gene-centric” product strategy. Our qSTAR qPCR primer pairs are designed for SYBR Green-based real-time qPCR. The primers are meticulously designed using OriGene's proprietary primer design algorithm developed from > 10,000 qPCR experiments. We are now offering the pre-validated qPCR primers and template standards for SARS-CoV-2 RdRP, N and S genes (below).

    Relative positions of amplicon targets on SARS-CoV-2 (previous Wuhan-CoV) genome for virus RdRp, N and S genes. Relative positions of amplicon targets on SARS-CoV-2 (previous Wuhan-CoV) genome. ORF: open reading frame; RdRp: RNA-dependent RNA polymerase; S: surface glycoprotein; N: nucleocapsid. Numbers below amplicon are genome locations corresponding to Wuhan-CoV genome, MN908947.

    SARS-CoV-2 Virus qPCR Quantification
    Protein Catalog# Product Description
    N HP234774 qSTAR qPCR primer pairs against SARS-CoV-2 N protein gene
    HK212669 qPCR primer pairs and template standards against SARS-CoV-2 N protein gene
    S HP234775 qSTAR qPCR primer pairs against SARS-CoV-2 S protein gene
    HK212670 qPCR primer pairs and template standards against SARS-CoV-2 S protein gene
    RdRp HP234776 qSTAR qPCR primer pairs against SARS-CoV-2 RdRp protein gene
    HK212671 qPCR primer pairs and template standards against SARS-CoV-2 RdRp protein gene

    1st Strand cDNA

    qPCR Primers

    qPCR Master Mix