MolBio Tools for COVID-19 Research (SARS-CoV-2 infection)
COVID19 is caused by a newly identified coronavirus named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Early studies have shown SARS-CoV-2 attaches to the human cells through an interaction between viral “Spike” protein and human ACE2 receptors; after binding to the cell surface, the “Spike” protein gets cleaved by human TMPRSS2 protease to facilitate the entry of virus.
To help researchers further dissect the molecular mechanism of this new virus, OriGene has developed various MolBio products covering all the SARS-CoV-2 virus genes and many human genes that play important roles in the pathogenesis of the virus.

Gene centric MolBio tools for COVID-19 research
Clones | RNAi | CRISPR kits | qPCR
Receptor & Activator
- ACE2 Receptor
Angiotensin-converting enzyme 2 (ACE2) is a membrane protein that has been found to play an important role in the infection of SARS-CoV-2 virus. The protein contains 805 amino acids with a N-terminal signal peptide, a transmembrane domain and a cytoplasmic domain. It is expressed in many tissues of human body, which may address the reason of non-respiratory symptoms seen in some COVID-19 patients . To many researchers, ACE2 has become a hot target due to the recent outbreak of COVID-19.
- Human
ACE2 MolBio Products
Human ACE2 MolBio Toolset Application Catalog# Product Description Overexpress ACE2
proteinin Mammalian
cellsSC124099 Untagged cDNA clone encodes native ACE2 protein. RC208442 ORF clone encodes C-terminal Myc-DDK tagged ACE2 protein RG208442 ORF clone encodes C-terminal tGFP tagged ACE2 protein RC208442L1 Lenti ORF clone with C-terminal Myc-DDK tag RC208442L2 Lenti ORF clone with C-terminal mGFP tag RC208442L3 Lenti ORF clone with Myc-DDK tag and puro selection marker RC208442L4 Lenti ORF clone with mGFP tag and puro selection marker RC208442L1V Lentiviral particle made from RC208442L1 RC208442L2V Lentiviral particle made from RC208442L2 RC208442L3V Lentiviral particle made from RC208442L3 RC208442L4V Lentiviral particle made from RC208442L4 Knockdown
ACE2 expression at
mRNA levelTR306881 Retroviral shRNA DNA including 4 shRNA & 1 scrambled control TL306881 Lentivrial shRNA DNA including 4 shRNA & 1 scrambled control TL306881V Lentiviral shRNA particles made from TL306881 SR311790 siRNA to knockdown the ACE2 expression Genome Editing KN408442 CRISPR gene knockout kit for human ACE2 GA111814 CRISPR gene activation kit for human ACE2 qPCR Quantification HP214223 qPCR primer pair for quantification of human ACE2 cDNA level HK212668 qPCR template standard for the calculation of exact copy number - Mouse
ACE2 MolBio Products
Mouse ACE2 MolBio Toolset Application Catalog# Product Description Overexpress ACE2
proteinin Mammalian
cellsMC221763 Untagged cDNA clone encodes native ACE2 protein. MR210755 ORF clone encodes C-terminal Myc-DDK tagged ACE2 protein MG210755 ORF clone encodes C-terminal tGFP tagged ACE2 protein MR210755L3 Lenti ORF clone with Myc-DDK tag and puro selection marker MR210755L4 Lenti ORF clone with mGFP tag and puro selection marker MR210755L3V Lentiviral particle made from MR210755L3 MR210755L4V Lentiviral particle made from MR210755L4 Knockdown
ACE2 expression at
mRNA levelTR504379 Retroviral shRNA DNA including 4 shRNA & 1 scrambled control TL504379 Lentivrial shRNA DNA including 4 shRNA & 1 scrambled control TL504379V Lentiviral shRNA particles made from TL504379 SR420297 siRNA to knockdown the ACE2 expression Genome Editing KN500711 CRISPR gene knockout kit for mouse ACE2 GA208393 CRISPR gene activation kit for mouse ACE2 qPCR Quantification MP220690 qPCR primer pair for quantification of mouse ACE2 cDNA level - Rat
ACE2 MolBio Products
Rat ACE2 MolBio Toolset Application Catalog# Product Description Overexpress ACE2
proteinin Mammalian
cellsRN214151 Untagged cDNA clone encodes native ACE2 protein. RR214151 ORF clone encodes C-terminal Myc-DDK tagged ACE2 protein RR214151L3 Lenti ORF clone with Myc-DDK tag and puro selection marker RR214151L4 Lenti ORF clone with mGFP tag and puro selection marker RR214151L3V Lentiviral particle made from RR214151L3 RR214151L4V Lentiviral particle made from RR214151L4 Knockdown
ACE2 expression at
mRNA levelTR701518 Retroviral shRNA DNA including 4 shRNA & 1 scrambled control TL701518 Lentivrial shRNA DNA including 4 shRNA & 1 scrambled control TL701518V Lentiviral shRNA particles made from TL701518 SR513861 siRNA to knockdown the ACE2 expression
- Human
ACE2 MolBio Products
- TMPRSS2
protease
Many coronaviruses including HCoV-229E, MERS-CoV, SARS-CoV and SARS-CoV-2 (COVID-19 virus) are found to use the same protease TMPRSS2 for the entry of the virus. TMPRSS2 is a serine protease that can cleave and activate the S-protein of the virus, which leads to the entry of the virus. Two spliced variants encoding different isoforms have been found for this gene.
- Human
TMPRSS2 Variant 1
Human TMPRSS2 Variant 1 MolBio Toolset Application Catalog# Product Description Overexpress TMPRSS2
isoform 1 protein (long
isoform) in Mammalian
cellsSC327067 Untagged cDNA clone encodes native TMPRSS2 isoform 1 protein RC228432 ORF clone encodes C-terminal Myc-DDK tagged TMPRSS2 isoform 1 protein RG228432 ORF clone encodes C-terminal tGFP tagged TMPRSS2 isoform 1 protein RC228432L1 Lenti ORF clone with C-terminal Myc-DDK tag RC228432L2 Lenti ORF clone with C-terminal mGFP tag RC228432L3 Lenti ORF clone with Myc-DDK tag and puro selection marker RC228432L4 Lenti ORF clone with mGFP tag and puro selection marker RC228432L1V Lentiviral particle made from RC228432L1 RC228432L2V Lentiviral particle made from RC228432L2 RC228432L3V Lentiviral particle made from RC228432L3 RC228432L4V Lentiviral particle made from RC228432L4 3’ UTR Clone
miRNA regulationSC215536 3’UTR clone to study the miRNA regulation of TMPRSS2 expression Knockdown
TMPRSS2 expression
at mRNA level (work for both isoforms)TR308745 Retroviral shRNA DNA including 4 shRNA & 1 scrambled control TL308745 Lentivrial shRNA DNA including 4 shRNA & 1 scrambled control TL308745V Lentiviral shRNA particles made from TL308745 SR322062 siRNA to knockdown the TMPRSS2 expression Genome Editing KN408677 CRISPR gene knockout kit for human TMPRSS2 GA104914 CRISPR gene activation kit for human TMPRSS2 qPCR Quantification HP208758 qPCR primer pair for quantification of human TMPRSS2 cDNA level HK207474 qPCR template standard for the calculation of exact copy number - Human
TMPRSS2 Variant 2
Human TMPRSS2 Variant 2 MolBio Toolset Application Catalog# Product Description Overexpress TMPRSS2
isoform 2 protein (short
isoform) in Mammalian
cellsSC323858 Untagged cDNA clone encodes native TMPRSS2 isoform 2 protein RC208677 ORF clone encodes C-terminal Myc-DDK tagged TMPRSS2 isoform 2 protein RG208677 ORF clone encodes C-terminal tGFP tagged TMPRSS2 isoform 2 protein RC208677L1 Lenti ORF clone with C-terminal Myc-DDK tag RC208677L2 Lenti ORF clone with C-terminal mGFP tag RC208677L3 Lenti ORF clone with Myc-DDK tag and puro selection marker RC208677L4 Lenti ORF clone with mGFP tag and puro selection marker RC208677L1V Lentiviral particle made from RC208677L1 RC208677L2V Lentiviral particle made from RC208677L2 RC208677L3V Lentiviral particle made from RC208677L3 RC208677L4V Lentiviral particle made from RC208677L4 3’ UTR Clone
miRNA regulationSC215537 3’UTR clone to study the miRNA regulation of TMPRSS2 expression Knockdown
TMPRSS2 expression
at mRNA level (work for both isoforms)TR308745 Retroviral shRNA DNA including 4 shRNA & 1 scrambled control TL308745 Lentivrial shRNA DNA including 4 shRNA & 1 scrambled control TL308745V Lentiviral shRNA particles made from TL308745 SR322062 siRNA to knockdown the TMPRSS2 expression Genome Editing KN408677 CRISPR gene knockout kit for human TMPRSS2 GA104914 CRISPR gene activation kit for human TMPRSS2 qPCR Quantification HP208758 qPCR primer pair for quantification of human TMPRSS2 cDNA level HK207474 qPCR template standard for the calculation of exact copy number - Mouse
TMPRSS2 MolBio Products
Mouse TMPRSS2 MolBio Toolset Application Catalog# Product Description Overexpress TMPRSS2
protein in Mammalian cellsMC204660 Untagged cDNA clone encodes native TMPRSS2 protein. MR207852 ORF clone encodes C-terminal Myc-DDK tagged TMPRSS2 protein MG207852 ORF clone encodes C-terminal tGFP tagged TMPRSS2 protein MR207852L3 Lenti ORF clone with Myc-DDK tag and puro selection marker MR207852L4 Lenti ORF clone with mGFP tag and puro selection marker MR207852L3V Lentiviral particle made from MR207852L3 MR207852L4V Lentiviral particle made from MR207852L4 Knockdown
TMPRSS2 expression at
mRNA levelTR502817 Retroviral shRNA DNA including 4 shRNA & 1 scrambled control TL502817 Lentivrial shRNA DNA including 4 shRNA & 1 scrambled control TL502817V Lentiviral shRNA particles made from TL502817 SR415788 siRNA to knockdown the TMPRSS2 expression Genome Editing KN517940 CRISPR gene knockout kit for mouse TMPRSS2 qPCR Quantification MP217322 qPCR primer pair for quantification of mouse TMPRSS2 cDNA level MK203882 qPCR template standard for the calculation of exact copy number - Rat
TMPRSS2 MolBio Products
Rat TMPRSS2 MolBio Toolset Application Catalog# Product Description Overexpress TMPRSS2
protein in Mammalian cellsRN214298 Untagged cDNA clone encodes native TMPRSS2 protein. RR214298 ORF clone encodes C-terminal Myc-DDK tagged TMPRSS2 protein RR214298L3 Lenti ORF clone with Myc-DDK tag and puro selection marker RR214298L4 Lenti ORF clone with mGFP tag and puro selection marker RR214298L3V Lentiviral particle made from RR214298L3 RR214298L4V Lentiviral particle made from RR214298L4 Knockdown
TMPRSS2 expression at
mRNA levelTR712521 Retroviral shRNA DNA including 4 shRNA & 1 scrambled control TL712521 Lentivrial shRNA DNA including 4 shRNA & 1 scrambled control TL712521V Lentiviral shRNA particles made from TL712521
- Human
TMPRSS2 Variant 1
SARS-CoV-2 Virus Genes
qPCR is the gold standard for gene expression analysis. It is a critical part of OriGene’s “gene-centric” product strategy. Our qSTAR qPCR primer pairs are designed for SYBR Green-based real-time qPCR. The primers are meticulously designed using OriGene's proprietary primer design algorithm developed from > 10,000 qPCR experiments. We are now offering the pre-validated qPCR primers and template standards for SARS-CoV-2 RdRP, N and S genes (below).

SARS-CoV-2 Virus qPCR Quantification | ||
---|---|---|
Protein | Catalog# | Product Description |
N | HP234774 | qSTAR qPCR primer pairs against SARS-CoV-2 N protein gene |
HK212669 | qPCR primer pairs and template standards against SARS-CoV-2 N protein gene | |
S | HP234775 | qSTAR qPCR primer pairs against SARS-CoV-2 S protein gene |
HK212670 | qPCR primer pairs and template standards against SARS-CoV-2 S protein gene | |
RdRp | HP234776 | qSTAR qPCR primer pairs against SARS-CoV-2 RdRp protein gene |
HK212671 | qPCR primer pairs and template standards against SARS-CoV-2 RdRp protein gene |