Validate Your Plasmid’s Protein Expression with a Complimentary Anti-DDK Antibody

Now you can quickly test the expression of your DDK-tagged clones with a complimentary sample of our new rabbit recombinant anti-DDK antibody (Cat # TA592569Sample). Order this free sample with your clone purchase using the code, DDK-Clone, at checkout. Offer valid until September 30^th, 2023.

How to claim your free sample to validate your plasmid:

• Add a DDK-tagged clone to your cart/PO
• Add the SKU TA592569Sample to your cart/PO
• Use code: DDK-Clone at checkout.

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Why Should I Use OriGene Clones?

• Sequence-verified
• Plasmids arrive transfection-ready (10 ug)
• Next day shipping available on select plasmids
• Proven to work: OriGene's clones have been successfully cited in Nature, Cell, and more! (view citations)

Figure 1. pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag
(Cat # PS100001)

Product Features

Product Name: p53 (TP53) (NM_000546) Human Tagged ORF Clone

Size: 10ug

E. Coli Selection: Kanamycin (25ug/mL)

Mammalian Cell Selection: Neomycin

Restriction Sites: SgfI-MluI

View this plasmid

TP53 activity in the over-expression lysate (LY400186) was measured in a colorimetric DNA-binding assay. Double-stranded oligonucleotide containing the p53 consensus DNA-binding sequence was incubated with dilutions of the over-expression lysate and TP53 bound to the oligo was captured onto the surface of a microtiter plate. After washing, bound TP53 was detected with an anti-p53 primary antibody followed by an HRP-labeled secondary antibody. After initial color development, the reaction was quenched and the color intensity was measured at 450nm. Overexpression cell lysates are prepared from HEK293T cells transfected with RC200003 using transfection reagent MegaTran 2.0 (Cat# [TT210002]).

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TP53 (Cat# RC200003, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TP53 (Cat# TA502870). Positive lysates LY400186 (100ug) and LC400186 (20ug) can be purchased separately from OriGene.

Western blot validation of overexpression lysate (Cat# LY400186) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200003 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Coomassie blue staining of purified TP53 protein (Cat# TP300003). The protein was produced from HEK293T cells transfected with TP53 cDNA clone (Cat# RC200003) using MegaTran 2.0 (Cat# TT210002).

Anti-TP53 mouse monoclonal antibody (TA502870) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TP53 (RC200003).

HEK293T cells transfected with either RC200003 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TP53 antibody (TA502870), and then analyzed by flow cytometry.

Terms and conditions

• This promotion is open to worldwide customers
• This promotion applies to online and offline orders from OriGene Technologies Inc or a qualifying distributor.
• This offer cannot be combined with any other ongoing promotions
• Offer valid only when using promo code. DDK-Clone with the purchase of a DDK-tagged clone.
• Offer valid till Sept 30^th or till the supplies last, whichever comes first.
• OriGene Technologies, Inc reserves all rights to disqualify, reject or terminate any order of the free sample as they deem fit
• Offer void where prohibited, licensed, or restricted by federal, state, or local laws or regulations or agency/institutional policy
• Other conditions may apply