Transfection Reagents

Researchers need to do gene overexpression (cDNA clones), gene knockdown (siRNA or shRNA) and gene knockout (CRISPR) to study gene functions. Effectively deliver those gene related products into cells is critical for their research. OriGene provides a broad range of transfection reagents for all of these different gene formats.

Selection Guide

Applications Transfection Reagents Features & Cell Types
Plasmid DNA Turbofectin 8.0™
  • Superior performance, comparable with FuGeneR 6
  • Broad spectrum for commonly used established cell lines, such as Hela, HEK293, MCF, 3T3, COS7, A549, etc.
Viromer RED,
Viromer PLASMID
  • Virus-like polymer
  • Active endosome escape leading to high transfection efficiency
  • Best for hard-to-transfect and primary cells, such as human primary macrophages, primary cardiomyocytes, MEF, THP1, HACAT etc. Click to see customer's data
MegaTran™ New
  • Economical choice
  • Excellent transfection efficiency
  • Broad cell spectrum (MegaTran 2.0)
293Tran™ New
  • Formulated specially for HEK293 transfection
  • High transfection efficiency
  • Low cytotoxicity
siRNA siTran™ New
  • High efficiency and low cytotoxicity
  • Commonly used cell lines and primary cells, such as human primary T cells
Viromer BLUE,
Viromer GREEN
  • Virus-like polymer
  • Active endosome escape leading to high transfection efficiency
  • Best for hard-to-transfect and primary cells, such as CACO-2, Kasumi-4, SH-SY5Y cell lines and human primary macrophages, monocytes and neurons, etc. Click to see customer's data
mRNA Viromer RED,
Viromer mRNA
  • Virus-like polymer
  • Active endosome escape leading to high transfection efficiency
  • Tested in difficult-to-transfect and primary cells, such as human primary monocytes, macrophages and dendritic cells
CRISPR RNP Viromer CRISPR
  • Efficient CRISPR RNP (gRNA and Cas9 protein complex) delivery
  • Superior transfection for genome editing
  • Higher reliability and efficiency of final editing compared to lipofection or electroporation
Lentivirus Production LentiTran™ New
  • Exceptional high titer lentivirus production
  • Simple & robust transfection procedure
  • Very affordable
  • TurboFectin 8.0™ - Lipid/histone based transfection reagent
    TurboFectin is a new generation of transfection reagent for nucleic acid delivery into eukaryotic cells. TurboFectin 8.0 is the recommended for the delivery of TrueClone/TrueORF clones (for over-expression) and HuSH-29 constructs (shRNA for expression knock-down) in many established cell lines and cell types.
    • Superior performance: Comparable with FuGENE®6 in trasfection efficiency and low cytotoxicity
    • Wide spectrum: Over 100 cell lines and primary cell types have been successfully transfected with TurboFectin 8.0. Click to see the list of cells tested
    • Affordability: Lower cost than FuGENE®6
  • MegaTran - Polymer based transfection reagent
    MegaTran 2.0 is a polymer based transfection reagent specially designed and manufactured for high volume DNA transfection and large scale protein production in a broad range of cells.
    • Superior transfection efficiency and low toxicity
    • Improved protein production via transient transfection
    • Cost effective compared to other commonly used transfection reagent

  • siTran – For transfection of the siRNA duplex
    siTran is a brand-new transfection reagent specially for siRNA duplexes in transient transfection, high-throughput screening, etc. siTran 1.0 is also highly effective in transfecting plasmid DNA.
    • Dual purpose reagent for both siRNA duplexes and plasmid DNA
    • High efficiency and low cytotoxicity
    • Has been successfully used in the transfection of T cells

  • Viromer – Virus-like polymer – Active escape transfection reagent
    Viromers, a technology breakthrough, take advantage of a viral fusion mechanism (hence their name) and translate this into polymer chemistry for plasmid and siRNA transfection.
    • Superior transfection efficiency, active endosome escape, maximizes transfection efficacy and reduces background
    • High efficiency and low cytotoxicity
    • Lipid-free, no interaction with cell metabolism in particular with lipid metabolism
    • Reverse Transfection, ready for High-throughput Screening

  • 293Tran – Specially formulated for transfecting HEK293 cells
    293Tran, which was formulated specifically for HEK293 cells for high transfection efficiency. 293Tran is a polymer-based and biodegradable transfection reagent.
    • Specialized for HEK293 – Maximize transfection performance in HEK293 cells
    • High Delivery Efficiency – Achieve 75-85% transfection efficiency
    • Low Cytotoxicity – Maintain cell density and reduce experimental biases

  • LentiTran – Specially formulated for high titer lentivirus production
    LentiTran is a polymer based transfection reagent that was developed for high titer lentivirus production in HEK293T cells.
    • Exceptional high titer lentivirus production
    • Simple & robust transfection procedure
    • Cost Effective