Please enter A/U/G/C's for RNA synthesis. OriGene recommends 3-5 sgRNA per target gene.
Enter the 17-20 nucleotide protospacer domain (your target sequence) directly into the order page as an RNA oligo
Please enter the sequence in a forward orientation so that the PAM site is downstream of your sequence, and do not
the PAM site. The ordering system will automatically add an 80-mer scaffold to your sequence to form a functional