Synthetic single guide (sgRNA) for efficient genome editing

Pure and simple

CRISPR application requires 2 key functional elements: target-specific guide RNA and CAS9 enzyme. Scientists have created gRNA using multiple methods, such as transfection of gRNA-expression plasmids, in vitro transcription, or by annealing a short crRNA oligo with a tracrRNA scaffold.

Recently, synthetic single guide has been recognized as the preferred way for highly efficient and accurate editing. The synthetic single gRNA is a pure 100-mer RNA oligo that contains the target gRNA sequence and the tracrRNA scaffold in a single entity.

Benefits of synthetic sgRNA (100mer):

  • Simple workflow
    Ready-to-use solution
    No in vitro transcription (as in IVT)
    No annealing/purification (as in the 2-oligo system)
  • Better in vivo stability
    No worry for RNase carry-over from the source
  • 100% DNA-free
    No risk of integrating foreign DNA into cell line
  • Better efficiency
    Up to 90% genome editing efficiency
  • Cost-effective
    Highly scalable for large numbers of experiments
  • Fast delivery
    sgRNA shipped out in 5-7 days
Sythetic vs IVT sgRNA The editing efficiency (solid bars) and consistency (error lines) of synthetic sgRNAs against in vitro transcribed (IVT) guide RNAs in HEK293T cells. The experiment was conducted by a third-party using three different gene targets and was replicated three times.

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How can sgRNA be used?

  • Transfection of sgRNA into a CAS9-expression cell line
  • Transfection of RNP of sgRNA/CAS9 protein into target cells
  • Transfection of sgRNA and a CAS9-expression plasmid into the target cells
  • Transfection of sgRNA and CAS9 mRNA into the target cells
  • Microinjection of sgRNA (together with Cas9 protein or mRNA) into embryo/cells

What will we deliver?

  • Synthetic CRISPR sgRNA (1nmol)
  • Sufficient for 10-20 Transfections
  • QC through Mass Spectrometry

How to order

  • Click on the quote button and input a 17-20nt target sequence for each sgRNA
  • OriGene recommends 3-5 sgRNA per target gene.
  • Place order via email or phone call (1.888.267.4436)
  • Contact us for modified RNA option or volume discount

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