Pure and simple
CRISPR application requires 2 key functional elements: target-specific guide RNA and CAS9 enzyme. Scientists have created gRNA using multiple methods, such as transfection of gRNA-expression plasmids, in vitro transcription, or by annealing a short crRNA oligo with a tracrRNA scaffold.
Recently, synthetic single guide has been recognized as the preferred way for highly efficient and accurate editing. The synthetic single gRNA is a pure 100-mer RNA oligo that contains the target gRNA sequence and the tracrRNA scaffold in a single entity.
Benefits of synthetic sgRNA (100mer):
- Simple workflow
No in vitro transcription (as in IVT)
No annealing/purification (as in the 2-oligo system)
- Better in vivo stability
No worry for RNase carry-over from the source
- 100% DNA-free
No risk of integrating foreign DNA into cell line
- Better efficiency
Up to 90% genome editing efficiency
Highly scalable for large numbers of experiments
- Fast delivery
sgRNA shipped out in 5-7 days
How can sgRNA be used?
- Transfection of sgRNA into a CAS9-expression cell line
- Transfection of RNP of sgRNA/CAS9 protein into target cells
- Transfection of sgRNA and a CAS9-expression plasmid into the target cells
- Transfection of sgRNA and CAS9 mRNA into the target cells
- Microinjection of sgRNA (together with Cas9 protein or mRNA) into embryo/cells
What will we deliver?
- Synthetic CRISPR sgRNA (1nmol)
- Sufficient for 10-20 Transfections
- QC through Mass Spectrometry
How to order
- Click on the quote button and input a 17-20nt target sequence for each sgRNA
- OriGene recommends 3-5 sgRNA per target gene.
- Place order via email or phone call (1.888.267.4436)
- Contact us for modified RNA option or volume discount
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