AAVS1 Transgene Insertion

Targeted Transgene Insertion into the AAVS1 Locus

Adeno-associated virus integration site 1 (AAVS1) in human genome is a safe harbor for transgene integration. It is transcriptionally active and transgene expression from this site is robust and stable. CRISPR technology is ideal for targeted transgene insertion at the AAVS1 locus.

  • Targeted transgene insertion
  • Robust and stable expression
  • No random genetic interruption and insertational mutagenesis
  • Genome-wide TrueORF collection serves as the source for transgenes
  • Easy shuttling from TrueORF clones into AAVS1 donor vector (Precision Shuttling System)

AAVS1 Safe Harbor knockin system via CRISPR

This system needs two components:

  1. pCas-Guide-AAVS1, All-in-one CRISPR vector for AAVS1 targeting
  2. pAAVS1-Puro-DNR, donor vector for transgene integration, containing the AAVS1 homologous arms (Gene of interest needs to be cloned)
AAVS1_simple_diagram

AAVS1 gRNA Vector

Cat # Product Name Description Manual
GE100023 pCas-Guide-AAVS1 AAVS1 gRNA vector, validated AAVS1 targeting sequence cloned in pCas-Guide vector

AAVS1 Donor Vector

Cat # Product Name Description Manual
GE100024 pAAVS1-Puro-DNR Donor vector with AAVS1 homologous arms cloned, ready for transgene cloning
GE100035 pAAVS1-BSD-DNR donor vector with AAVS1 homologous arms cloned (Blasticidin selection), ready for exogenous gene cloning (10 ug)
GE100046 pAAVS1-EF1a-puro-DNR AAVS1 donor vector with AAVS1 homologous arms cloned (Puro selection), EF1a promoter, ready for exogenous gene cloning (10 ug)
GE100048 pAAVS1-EF1a-BSD-DNR AAVS1 donor vector with AAVS1 homologous arms cloned (Blasticidin selection), EF1a promoter, ready for exogenous gene cloning (10 ug)

Transgene Knockin Vector Kits

Cat # Product Name Description Manual
GE100027 AAVS1 Transgene knockin vector kit Vector kit for targeted transgene insertion into AAVS1 locus, including GE100023, GE100024 and GE100003
GE100036 AAVS1 transgene knockin kit (BSD) Vector kit for targeted transgene insertion into AAVS1 locus (blasticidin selection), including GE100023, GE100035 and GE100003
GE100047 AAVS1 Transgene Knockin kit (EF1a, Puro) Vector kit for targeted transgene insertion into AAVS1 locus (EF1a, puro), including GE100023, GE100046 and GE100003
GE100049 AAVS1 Transgene Knockin kit (EF1a, BSD) Vector kit for targeted transgene insertion into AAVS1 locus (EF1a, blasticidin), including GE100023, GE100048 and GE100003

Scheme of AAVS1 transgene knockin via CRISPR

AAVS1_simple_diagram

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Gene Expression Resources