Lentiviral Products and Services

What is Lentivirus?

Lentivirus is a genus of retroviruses that express reverse transcriptase and integrase. These two enzymes are used to integrate viral DNA into host DNA, allowing for the exploitation of host machinery to express viral genes. Lentiviral vectors are modified from HIV-1, with many of the viral genes removed. The lentiviral vector only contains the LTRs and the packaging signal, Ψ. In addition, lentiviral packaging genes are provided on separate plasmids, so the pseudo lentiviral particles are replication deficient. Using lentiviral vectors to deliver genes into targeted cells has proven to be a dependable, efficient, and safe method for research.

Benefits of Using Lentivirus

• Broad cell spectrum: Lentivirus can infect many cells including dividing & non-dividing, easy-to-transfect, and hard-to-transfect cells.
• High transduction efficiency.
• Convenience: Minimal need for optimization.
• Safety: 3^rd generation system with improved biosafety.

Lentiviral System Validation Data

Lenti-ORF

Tagged LentiORF
clones/particles

Lenti-shRNA

Lentiviral shRNA
particles for gene
silencing

Lentiviral Accessories

From packaging kits
to transfection
reagents

Custom Lentivirus Production

Small and large-
scale production

Lentiviral CRISPR Vectors

For gRNA and Cas9
expression

What makes our lentiviral particles special?

• Pre-titered, ready-to-use
• Titer guaranteed, >10^7 TU/mL
• Provided in the proprietary Lenti Stabilizer Solution with >1 year infectivity
• Trusted vendor for lentiviral products: view citations

To learn more about how to make lentivirus, watch our video on lentivirus production. This video has all the information you need to produce high quality lentiviral particles, from lentivirus packaging, virus titration, and virus concentration to particle stabilization.

Lentiviral System Resources

• Lenti protocol
• Recommended lentivirus MOI for common cell lines
• Does lentivirus work with neurons?
• Frequently asked questions about lenti
• How to make lentivirus?
• Webinar: How to make lentivirus
• Lenti webinar
• Lenti biosafety

Customer Reviews

"I have used lenti-ORF plasmids for the generation of overexpression stable cells. They work well. The cell selection marker in the vector made it easier to generate stable cells."

Review by A.J.

Medical University of South Carolina
 

"The ready-to-use lentivirus particles worked well in our hard-to- transduce cell lines."

Review by K.W.

University Hospital of Tuebingen, Children's Hospital

"I purchased a lenti-ORF from OriGene. I have good transfection and transduction efficiency with OriGene's lenti products. Overall, I am extremely satisfied."

Review by S.R.

Sidney Kimmel Cancer Center
 

"I used both the lentivirus and the lenti plasmids. Both of them worked very well. And the customer service is good too."

Review by B.S.

St. Jude Children's Research Hospital
 

"We Purchased multiple lenti vectors from OriGene and they all worked great."

Review by S.D.

MD Anderson Cancer Center
 

"Aml12 cells are very difficult to transfect using siRNAs. Hence, using OriGene's lenti-shRNA product helped tackle this issue."

Review by Y.L.

University of Queensland
 

"We as research labs always look for an efficient and reliable lentiviral products and OriGene has really done a great job for past several years for us. We are extremely satisfied with the product's efficacy of knockdown, literature provided and technical support when needed. On top of this the service was fast enough. I shall highly recommend researchers to go for OriGene. Thank you."

Review by A.G.

Moffitt Cancer Center
 

"Our lab tried to package our lentivirus using 293T cells but it was not that easy. OriGene's lentivirus provided us consistent results which made us have clear idea about the further research goals. The purchases from OriGene have been worth the price."

Review by H.T.

University of Arkansas for Medical Sciences

"I have tried lentivirus expressing shRNA and it was working to my expectation. I am very happy with the product."

Review by G.P.

Centre for Stem Cell Research
 

"We used lentiviruses to silence Pmch gene and it worked beautifully."

Review by M.F.

USP
 

"Great quality, good knock-down. Super satisfied."

Review by C.H.

Cambridge University