The Multi-Fragment DNA Assembly Mix is a simple, fast, and efficient DNA seamless cloning method that connects multiple overlapping DNA fragments into a joined DNA molecule without the use of restriction enzyme cloning. This product can be used for the simultaneous assembly of up to five different inserts with a linearized vector in a single tube reaction, regardless of compatible digestion sites. The final products are fully ligated DNA constructs that are ready to transform into competent cells or used in further PCR or RCA cloning application. The buffer components and enzymes in the 2X Master Mix are optimized to enhance the efficiency and robustness of assembly reaction.
|NP100054||The Multi-fragment DNA Assembly Mix (2X) is a simple, fast and high-quality cloning technology, which can clone inserts to any vector in a targeted manner.||10 Rxn
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