pCas-Guide-Puro-CRISPRi-Scramble, scramble gRNA control in GE100083 vector (with Puro selection)
Vectors
View as table DownloadHuSH shRNA RFP Lenti Cloning Vector (pRFP-C-shLenti Vector), 5 ug
Viral Packaging | Lentiviral |
pCas-Guide-Nickase vector for gRNA cloning (with Cas9 H840A expression) (10 µg)
Function | Cas9 Nickase |
pLenti-C-Myc-DDK-P2A-Puro-mGFP, Lenti vector with C-terminal Myc-DDK tag, P2A-Puro selection marker and mGFP reporter gene
C-Tag | Myc-DDK |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
pCMV6-AC-mRFP, mammalian vector with C-terminal mRFP, 10ug
C-Tag | mRFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Puromycin |
Catalytically inactive Cas9 (D10A/H840A) vector for gRNA cloning (10 µg)
Function | Cas9 Nickase |
pLenti-Cas9-Myc-DDK-P2A-Puro control plasmid containing the expression cassette for Cas9-Myc-DDK fusion protein and Puromycin resistance.
Function | Cas9 only vectors |
pLenti-EF1a-C-Myc-DDK-IRES-BSD, Lenti vector with bicistronic IRES-BSD, 10ug
C-Tag | Myc-DDK |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Blasticidin |
29-mer Scrambled shRNA Cassette in p-RFP-C-shLenti Vector, 5 ug
Viral Packaging | Lentiviral |
HuSH shRNA Lenti Cloning Vector (pC-shLenti Vector), 5 ug
Viral Packaging | Lentiviral |
pCMV6-AC-mGFP-Puro, mammalian vector with C-terminal mGFP, 10ug
C-Tag | mGFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Puromycin |
29-mer Scrambled shRNA Cassette in pGFP-A-shAAV Vector, 5ug
Viral Packaging | Adeno-associated virus (AAV) |
USD 1,150.00
2 Weeks
RFP negative transgenic cell lines (HEK293T cells containing RFP dead mutant at AAVS1 locus), can be edited to convert to RFP positive cells
USD 200.00
2 Weeks
Validated repairing donor oligos for RFP dead mutant correction in GE100041C cells
Validated sgRNA targeting RFP dead mutant in GE100041C cells
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Reporters
C-Tag
- Myc-DDK (23)
- mGFP (11)
- His (4)
- Tag Free (3)
- mRFP (3)
- tGFP (3)
- DDK-His (2)
- FC (2)
- GST (2)
- HA (2)
- mCFP (2)
- tRFP (2)
- 3 HA (1)
- 3DDK (1)
- DDK (1)
- GFP (1)
- HA-DDK (1)
- HA-His (1)
- IT10 (1)
- IT5 (1)
- IT6 (1)
- Luciferase (1)
- Myc (1)
- Myc-His (1)
- TurboFP602 (1)
- TurboFP635 (1)
- TurboGFP (1)
- TurboRFP (1)
- TurboYFP (1)
- mBFP (1)
- mKate (1)
- mYFP (1)
- tYFP (1)