RNAi

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Reporters:
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PRKY (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each

Purity HPLC purified
Number of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).

PRKY - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector, 5µg of each construct provided

Format Retroviral plasmids
Vector pRFP-C-RS
E. coli Selection Chloramphenicol (34 ug/ml)
Mammalian Cell Selection Puromycin

PRKY - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided

Format Lentiviral plasmids
Vector pGFP-C-shLenti
E. coli Selection Chloramphenicol (34 ug/ml)
Mammalian Cell Selection Puromycin

PRKY - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided

Format Retroviral plasmids
Vector pRS
E. coli Selection Ampicillin
Mammalian Cell Selection Puromycin