Semi purified Pyruvate Kinase from rabbit muscle. Myokinase 0.005% GPD, CPK, ATPase, NADH, phospho-glycerate-mutase all 0.001%. The amount of enzyme that produces one micromole of pyruvate per minute at pH 7.6 from PEP in the presence of ADP at 30°C.
Rabbit IgG whole molecular was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Rabbit IgG whole molecular was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Rabbit Serum.
This product is designed as a negative control reagent alongside specific FITC-conjugated rabbit IgG antibodies. It has been tested in flow cytometry on human tissues and demonstrates negligible binding.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum and anti-rabbit IgM (µ chain specific). No reaction was observed against anti rabbit IgG F(c). Some light chain cross-reactivity will occur with anti-rabbit IgG.