Endothelial cell growth factor (ECGF) is an extract of bovine brain containing growth promoting factors for vascular endothelial cells of mammalian origin. ECGF has also been reported to be beneficial as a media supplement for the fusion and growth of hybridoma cells in monoclonal antibody production. Endothelial cell growth factor is prepared using a modification of the method of Maciag, et al. (1979) lyophilized from a sterile solution containing NaCl and streptomycin sulfate.
ECGFpro1 is supplemented with recombinant human VEGF165 (corresponding to 10 ng/ml) a concentration sufficient for the cultivation of blood vascular endothelial cells like HUVEC and HMVEC.
Species specificity: Bovine ECGF is effective on Mouse, Bovine and Human cells.