Gene Expression

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Bglap - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated.

Format 2 gRNA vectors, 1 linear donor
Donor DNA EF1a-GFP-P2A-Puro

qSTAR qPCR primer pairs against Homo sapiens gene BGLAP

Component 1 vial of lyophilized qSTAR qPCR primer mix (1 nmol each primer, sufficient for 200 reactions)

qSTAR qPCR primer pairs against Mus musculus gene Bglap1

Component 1 vial of lyophilized qSTAR qPCR primer mix (1 nmol each primer, sufficient for 200 reactions)

BGLAP CRISPRa kit - CRISPR gene activation of human bone gamma-carboxyglutamate protein

Format 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)

Bglap CRISPRa kit - CRISPR gene activation of mouse bone gamma carboxyglutamate protein

Format 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)

qPCR primer pairs and template standards against Homo sapiens gene BGLAP

Application Plasmid of exact quantity for transcript copy number calculation