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Oligo annealing buffer (10X)
Gene Expression
View as table DownloadSensiMix SYBR master mix with Hi-ROX, 50 x 25 uL rxn
SensiMix SYBR master mix with Hi-ROX, 400 x 25 uL rxn
qSTAR qPCR primer pairs against Mus musculus gene Tnf
| Component | 1 vial of lyophilized qSTAR qPCR primer mix (1 nmol each primer, sufficient for 200 reactions) |
Cre recombinase mammalian expression vector
Forward primer to sequence targets cloned in expression vectors.
| Applicable Vectors |
PS100004,
PS100005,
PS100006,
PS100007,
PS100008,
PS100009,
PS100010,
PS100011,
PS100012,
PS100013,
PS100014,
PS100016,
PS100017,
PS100018,
PS100020,
PS100022,
PS100024,
PS100025,
PS100026,
PS100027,
PS100034,
PS100036,
PS100038,
PS100039,
PS100040,
PS100041,
PS100042,
PS100043,
PS100044,
PS100053,
PS100054,
PS100057,
PS100058,
PS100059,
PS100064,
PS100065,
PS100066,
PS100067,
PS100069,
PS100071,
PS100074,
PS100081,
PS100082,
PS100088,
PS100092,
PS100093,
PS100094,
PS100101,
PS100102,
PS100103,
PS100104,
PS100105,
PS100107,
PS100108,
PS100109,
PS100110,
PS100111,
PS100126,
PS100135,
PS100136,
PS100139
|
CF3, the forward sequencing primer to sequence target sequence cloned in CRISPR vectors.
SensiMix SYBR master mix with fluorescein, 400 x 25 uL rxn
Cas9 in vitro digestion buffer, 10x
PCR Primer mix to confirm AAVS1 integration, amplifying the left integration junction, 2 nmols
NLRP1 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
SensiMix SYBR master mix with Low-ROX, 400 x 25 uL rxn
CRISPR/Cas9 starter kit (HA tagging human HSP60 at C-terminus)
SensiMix SYBR master mix with fluorescein, 1600 x 25 uL rxn
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