Antibodies

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Human IgG1 (Fc specific) Mouse Monoclonal Antibody [Clone ID: NI 132 (HP 6186)]
AM20252BT-N

USD 865.00

2 Weeks

Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), Biotin

Applications To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG1, and Immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used.
General Recommended Dilutions:
Immunocytochemistry.
Immunohistochemistry (Paraffin).
Histochemical Use: 1/10-1/40.
ELISA: 1/100-1/2000. 
Western blot: 1/200-1/4000.
Reactivities Human
Conjugation Biotin
IGHG4 Mouse Monoclonal Antibody [Clone ID: HP6025]
SM2069AP

USD 425.00

5 Days

IGHG4 mouse monoclonal antibody, clone HP6025, AP, Purified

Applications ELISA: 1/1000-1/2000 (2-9).
Immunohistochemistry on Frozen Sections:  reported in literature (5,7,10,11).
Immunohistochemistry on Paraffin Sections: reported in literature (10).
Flow Cytometry: reported in literature (15, 18, 19).
Immunocytochemistry: reported in literature (12).
Western Blot: reported in literature (3,13-16).
Reactivities Human
Conjugation AP
Human IgA Mouse Monoclonal Antibody [Clone ID: NI 69 (A89-034) and NI 184 (A89-035)]
AM20249AF-N

USD 580.00

2 Weeks

Human IgA mouse monoclonal antibody, clone NI 69 (A89-034) and NI 184 (A89-035), Ascites

Applications To identify the presence of IgA in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining of cytoplasmic IgA, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200. 
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated
Human IgG2 (Fc specific) Mouse Monoclonal Antibody [Clone ID: 31-7-4]
AM08152HR-N

USD 410.00

3 Days

Human IgG2 (Fc specific) mouse monoclonal antibody, clone 31-7-4, HRP

Applications ELISA: 1/2,000-1/8,000.
Reactivities Human
Conjugation HRP
Human IgG1 (Fc specific) Mouse Monoclonal Antibody [Clone ID: NI 132 (HP 6186)]
AM20252HR-N

USD 865.00

2 Weeks

Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), HRP

Applications To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Direct Immunoperoxidase staining of cytoplasmic IgG1, and Immunoblotting.
General Recommended Dilutions:
ELISA: 1/20-1/500.
Western Blot: 1/40-1/1000. 
Histochemical Use: 1/5-1/20.
Reactivities Human
Conjugation HRP
Human IgG (Fd region) Mouse Monoclonal Antibody [Clone ID: HP6045]
AM31855HR-N

USD 525.00

2 Weeks

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, HRP

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay.
Reactivities Human
Conjugation HRP
Human IgG2 (Hinge Region) Mouse Monoclonal Antibody [Clone ID: 3C7]
BM480

USD 690.00

5 Days

Human IgG2 (Hinge Region) mouse monoclonal antibody, clone 3C7, Purified

Applications ELISA.
Reactivities Human
Conjugation Unconjugated
Mouse IgG2a (heavy chain) Rat Monoclonal Antibody [Clone ID: LO-MG2a-7]
SM1018F

USD 820.00

5 Days

Mouse IgG2a (heavy chain) rat monoclonal antibody, clone LO-MG2a-7, FITC

Applications Flow Cytometry: Use 10 µl of 2.5 µg/ml diluted antibody to label 106 cells in 100 µl.
Reactivities Mouse
Conjugation FITC
Rat IgA (heavy chain) Mouse Monoclonal Antibody [Clone ID: MARA-1]
SM1027P

USD 575.00

5 Days

Rat IgA (heavy chain) mouse monoclonal antibody, clone MARA-1, Purified

Applications ELISA: 5 µg/ml. This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 (Cat.-No AM05512HR-N) as detection reagent and purified Rat IgA (Cat.-No SA013) as antigen.
Immunofluorescence.
Immunohistochemistry on Frozen Sections.
Reactivities Rat
Conjugation Unconjugated
Bovine IgG (light chain) Mouse Monoclonal Antibody [Clone ID: IVA285-1]
AM03077PU-N

USD 245.00

2 Weeks

Bovine IgG (light chain) mouse monoclonal antibody, clone IVA285-1, Purified

Applications Western Blotting (Reducing Conditions): 0.5-1 μg/ml, overnight in 4°C.
SDS-PAGE (12% separating gel).
Positive Control and Sample Peparation: BS (Bovine Serum), dilution 1/50 in Laemmli reducing buffer, boiled in water bath for 3 min.
Strongly reacts with Bovine IgG light chains, weakly reacts with IgM.
Flow Cytometry.
Immunohistochemistry on Frozen Sections.
Reactivities Bovine, Sheep
Conjugation Unconjugated
Human IgE Mouse Monoclonal Antibody [Clone ID: BE5]
SM3061P

USD 205.00

2 Weeks

Human IgE mouse monoclonal antibody, clone BE5, Purified

Applications ELISA.
Flow Cytometry: 5-10 µg/ml.
Reactivities Human
Conjugation Unconjugated
Human IgM (Fc-region) Mouse Monoclonal Antibody [Clone ID: CH2]
SM3063P

USD 205.00

2 Weeks

Human IgM (Fc-region) mouse monoclonal antibody, clone CH2, Purified

Applications Western blot. Recommended dilution: 1 μg/ml.
Flow cytometry: Recommended dilution: 1-4 μg/ml, extracellular and intracellular staining.
Immunocytochemistry.
ELISA.
Reactivities Human
Conjugation Unconjugated
Human IgA (secretory) Mouse Monoclonal Antibody [Clone ID: SC-05]
SM3073P

USD 220.00

2 Weeks

Human IgA (secretory) mouse monoclonal antibody, clone SC-05, Purified

Applications Western blot.
Immunoprecipitation.
Immunohistochemistry on Paraffin Sections.
Immunohistochemistry on Frozen Sections.
Examples of positive Human tissues: Secretory mucosa of gastrointestinal and respiratory tract, epithelia of salivary glands, endometrium, endocervix, kidney, prostate, mammary gland.
Reactivities Human
Conjugation Unconjugated
Rat IgM (Mu chain specific) Mouse Monoclonal Antibody [Clone ID: M 2A1]
AM08095RP-N

USD 440.00

3 Days

Rat IgM (Mu chain specific) mouse monoclonal antibody, clone M 2A1, PE

Applications Flow Cytometry: (< / = 0.1 µg/10e6 cells) and Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells.
FLISA: < / = 1.0 µg/ml
Reactivities Rat
Conjugation PE
Human IgG (Fd region) Mouse Monoclonal Antibody [Clone ID: HP6045]
AM31855PU-N

USD 565.00

2 Weeks

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, Purified

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay
Reactivities Human
Conjugation Unconjugated