Antibodies

View as table Download

Bovine IgM (Fc specific) rabbit polyclonal antibody, Biotin

Applications Immunocytochemical and Immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
To demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases.
To identify a specific antigen using an reference antibody of bovine origin known to be of the IgM isotype in the middle layer of the indirect test procedure.
In non-isotopic assay methodology (e.g. ELISA) to measure IgM in Bovine serum or other body fluids.
As a second step an avidin or streptavidin conjugate of the user’s choice has to be used.
Recommneded Dilutions:
Histochemistry and Cytochemistry: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/100-1/500.
Reactivities Bovine
Conjugation Biotin

Bovine IgM (Fc specific) rabbit polyclonal antibody, HRP

Applications Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
To demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases.
To identify a specific antigen using an reference antibody of bovine origin known to be of the IgM isotype in the middle layer of the indirect test procedure.
In non-isotopic assay methodology (e.g. ELISA) to measure IgM in Bovine serum or other body fluids.
Recommneded Dilutions:
Histochemistry and Cytochemistry: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/2,000.
Reactivities Bovine
Conjugation HRP

Bovine IgM (Fc specific) rabbit polyclonal antibody, Azide Free

Applications This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase Immunochemistry.
Recommneded Dilutions:
Histochemistry: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/2,000.
Reactivities Bovine
Conjugation Unconjugated

Bovine IgM (Fc specific) rabbit polyclonal antibody, FITC

Applications Immunocytochemical and Immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
To demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases.
To identify a specific antigen using an reference antibody of bovine origin known to be of the IgM isotype in the middle layer of the indirect test procedure.
In non-isotopic assay methodology (e.g. ELISA) to measure IgM in Bovine serum or other body fluids.
Recommneded Dilutions: 1/20-1/80.
Reactivities Bovine
Conjugation FITC

Bovine IgG1 sheep polyclonal antibody, Aff - Purified

Applications ELISA.
Immunodiffusion.
Reactivities Bovine
Conjugation Unconjugated

Bovine IgG1 (subclass specific) rabbit polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG1 in bovine serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of bovine IgG1 from serum or plasma.
Antibody titre: Precipitin titre not less then 1/16 when tested against pooled normal bovine serum in agar block immunodiffusion titration.
Recommended Dilutions:
Immunoelectrophoresis: 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Reactivities Bovine
Conjugation Unconjugated

Bovine IgA (secretory) rabbit polyclonal antibody, Serum

Applications This antibody can be used in precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgA in Bovine serum, milk or other body fluids or to determine its concentration.
Can also be used to prepare an immunoadsorbent for the purification of Bovine IgA from serum or exocrine secretions.
Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms as secretory IgA.
This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. This does not exclude such use if proper controls are include.
Recommneded Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre not less than 1/32 when tested against pooled normal Bovine serum in agar block titration.
Reactivities Bovine
Conjugation Unconjugated

Bovine IgG (Fc specific) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgG in bovine serum or other body fluids or to determine its concentration.
To prepare an Immunoadsorbent for the purification of Bovine IgG from serum or plasma.
Recommended Dilutions:
Immunoelectrophoresis: Use 2 µl serum or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl serum samples (neat and serially diluted in 2 mm diameter peripheral wells.
Precipitin titre: 1/64 when tested against pooled normal bovine serum in agar-block Immunodiffusion titration.
Reactivities Bovine
Conjugation Unconjugated

Bovine IgG2 (subclass specific) rabbit polyclonal antibody, Serum

Applications This antibody can be used in precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgG2 in Bovine serum and other body fluids or to determine its concentration.
Can also be used to prepare an immunoadsorbent for the purification of bovine IgG2 from serum or plasma.
Recommneded Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre not less than 1/32 when tested against normal Bovine serum in agar block titration.
Reactivities Bovine
Conjugation Unconjugated

Bovine IgG2 (subclass specific) sheep polyclonal antibody, Serum

Applications This antibody can be used in precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgG2 in Bovine serum and other body fluids or to determine its concentration.
Recommneded Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre not less than 1/32 when tested against normal goat serum in agar block titration.
Reactivities Bovine
Conjugation Unconjugated

Bovine IgM (Fc specific) rabbit polyclonal antibody, Serum

Applications This antibody can be used in precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgM in Bovine serum and other body fluids or to determine its concentration.
Can also be used to prepare an immunoadsorbent for the purification of Bovine IgM from serum or plasma.
Recommneded Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre not less than 1/16 when tested against normal Bovine serum in agar block titration.
Reactivities Bovine
Conjugation Unconjugated

Bovine IgG sheep polyclonal antibody, Purified

Applications ELISA: 1/100-1/10,000.
Immunodiffusion.
Reactivities Bovine
Conjugation Unconjugated

Bovine IgM sheep polyclonal antibody, Aff - Purified

Applications ELISA: 1/100-1/1000.
Immunodiffusion.
Reactivities Bovine
Conjugation Unconjugated

Bovine IgG2 sheep polyclonal antibody, Aff - Purified

Applications ELISA: 1/100 - 1/10000.
Immunodiffusion.
Reactivities Bovine
Conjugation Unconjugated