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Mouse Monoclonal tGFP Antibody, Clone OTI2H8, Magnetic Beads

TA150039

USD 776.00

In Stock

Mouse Monoclonal tGFP Antibody, Clone OTI2H8, Magnetic Beads

Applications	IP, Protein Purification
Conjugation	Magnetic Beads

$Immunoprecipitation (IP) was performed with overexpression lysate expressed with tGFP expression vector (Cat# [PS100010]) or control lysate transfected with pCMV-Entry empty vector (Cat# [PS100001]). Briefly, 20uL anti-tGFP (2H8) magnetic beads (Cat# TA150039) were incubated with tGFP overexpression lysate or negative control lysate overnight at 4°C, respectively. After washing three times with RIPA buffer, 20uL 4x SDS sample buffer was applied to the lysate-beads mixture. The samples were boiled at 95°C for 10min. The IP sample supernatants were fractionated on SDS-PAGE gel, blotted onto nitrocellulose membrane. The IP efficiency was evaluated by using HRP-conjugated anti-tGFP antibody (Cat# [TA150043]). Lane 1: anti-tGFP (2H8) magnetic beads (20uL) with tGFP expression lysate; Lane 2: anti-tGFP magnetic beads (20uL) with negative control lysate.$

Mouse Monoclonal tGFP Antibody, Clone OTI2H8, Magnetic Beads

TA150039S

USD 470.00

In Stock

Mouse Monoclonal tGFP Antibody, Clone OTI2H8, Magnetic Beads

Applications	IP, Protein Purification
Conjugation	Magnetic Beads

$Immunoprecipitation (IP) was performed with overexpression lysate expressed with tGFP expression vector (Cat# [PS100010]) or control lysate transfected with pCMV-Entry empty vector (Cat# [PS100001]). Briefly, 20uL anti-tGFP (2H8) magnetic beads (Cat# [TA150039]) were incubated with tGFP overexpression lysate or negative control lysate overnight at 4°C, respectively. After washing three times with RIPA buffer, 20uL 4x SDS sample buffer was applied to the lysate-beads mixture. The samples were boiled at 95°C for 10min. The IP sample supernatants were fractionated on SDS-PAGE gel, blotted onto nitrocellulose membrane. The IP efficiency was evaluated by using HRP-conjugated anti-tGFP antibody (Cat# [TA150043]). Lane 1: anti-tGFP (2H8) magnetic beads (20uL) with tGFP expression lysate; Lane 2: anti-tGFP magnetic beads (20uL) with negative control lysate.$

1 - 2 of 2 results

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Antibodies (2)
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TGFP

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Mouse

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__Category ID

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13

2

201019

201023

201026

200664

201250

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