Antibodies

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Canine IgG (H+L chain) Goat Polyclonal Antibody
AP31541SU-N

USD 360.00

2 Weeks

Canine IgG (H+L chain) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG; as secondary antibody to precipitate the immunoglobulin in normal dog serum; to prepare an immunoadsorbent for the production of Ig-free dog serum, plasma or non-Ig preparations.
Directions for use:
In immunoelectrophoresis use 2 ml or equivalent against 120 ml antiserum.
In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ml antiserum in a 3 mm diameter centre well and 2 ml serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Reactivities Canine
Conjugation Unconjugated
Canine IgM (Fc specific) Goat Polyclonal Antibody
AP31542BT-N

USD 705.00

2 Weeks

Canine IgM (Fc specific) goat polyclonal antibody, Biotin

Applications Can be used in immunocytochemical and immunohistochemical use for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using an reference antibody of goat origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in dog serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/250.
In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/4000.
Reactivities Canine
Conjugation Biotin
Canine IgM (Fc specific) Goat Polyclonal Antibody
AP31542SU-N

USD 360.00

2 Weeks

Canine IgM (Fc specific) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in dog serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of dog IgM from serum or plasma.
Directions for use:
In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum.
In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl in a 3 mm diameter center well and 2 μl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
Reactivities Canine
Conjugation Unconjugated
Fibrinogen Rabbit Polyclonal Antibody
AP33055SU-N

USD 360.00

2 Weeks

Fibrinogen rabbit polyclonal antibody, Serum

Applications ID, IP
Reactivities Canine
Conjugation Unconjugated
Canine IgG (Fc specific) Sheep Polyclonal Antibody
AP33058SU-N

USD 360.00

2 Weeks

Canine IgG (Fc specific) sheep polyclonal antibody, Serum

Applications Immunoprecipitation.
Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG in dog serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of dog IgG from serum or plasma. The antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive techniques. For such uses labelled and unlabelled cytochemical grade reagents raised in goats meeting the necessary additional specificity and performance requirements are available.
Directions for Use:
Immunoelectrophoresis: Use 2 μl serum or equivalent against 120 μl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells. 
Precipitin titre: 1/16 when tested against pooled normal dog serum in agar-block immunodiffusion titration.
Reactivities Canine
Conjugation Unconjugated