Secondary Antibodies

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Feline IgG (H+L chain) goat polyclonal antibody, Serum

Applications Can be used:
• In precipitating techniques as Immunoelectrophoresis and Double Radial Immunodiffusion to identify the presence of IgG.
• As secondary antibody to precipitate the immunoglobulin in normal Cat serum.
• To prepare an immunoadsorbent for the production of Ig-free Cat serum, plasma or non-Ig preparations.
Directions for use:
Immunoelectrophoresis: Use 2 μl or equivalent against 120 μl antiserum. 
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Reactivities Feline
Conjugation Unconjugated

Feline IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Cat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:16,000 of the reconstitution concentration is suggested for this product.
Reactivities Feline
Conjugation HRP

Feline IgM goat polyclonal antibody, Purified

Applications ELISA.
Reactivities Feline
Conjugation Unconjugated

Monkey IgA mouse monoclonal antibody, clone IgA5-3B, Purified

Applications ELISA.
Western Blot: Detects a band of approximately 55kDa under reducing conditions.
Reactivities Feline, Monkey
Conjugation Unconjugated

Feline IgG mouse monoclonal antibody, clone GPB2-2B1, Purified

Applications ELISA: This antibody can bind to virus-bound immune IgG and can therefore detect anti-FeLV, FIV, FCV, FHV, FCoV/FIPV and FPV IgG activity in ELISA.
Western Blot: This antibody detects a band of approximately 50.0kDa on purified Feline IgG under reducing conditions.
Reactivities Feline
Conjugation Unconjugated

Feline IgA (Fc specific) goat polyclonal antibody, Serum

Applications Precipitating techniques as Immunoelectrophoresis and Single and Double Radial Immunodiffusion (Mancini, Ouchterlony) to identify the presence of IgA in Cat serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of Cat IgA from serum or plasma.
Recommended Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion: Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody Titre: Precipitin titre not less than 1/32 when tested against normal Cat serum in agar block titration.
Reactivities Feline
Conjugation Unconjugated

Feline IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Serum

Applications Precipitating techniques as Immunoelectrophoresis and Single and Double Radial Immunodiffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins; as secondary antibody to precipitate the immunoglobulin in normal Cat serum.
Recommended Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion: Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody Titre: Precipitin titre not less than 1/64 when tested against normal Cat serum in agar block titration.
Reactivities Feline
Conjugation Unconjugated

Feline IgA (secretory) goat polyclonal antibody, Serum

Applications Precipitating techniques as Immunoelectrophoresis and Single and Double Radial Immunodiffusion (Mancini, Ouchterlony) to identify the presence of IgA in Cat milk or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of Cat IgA from serum or exocrine secretions.
Antisera to IgA or sIgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. This does not exclude the use of the antiserum in more sensitive techniques if proper controls are included.
Recommended Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion: Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody Titre: Precipitin titre not less than 1/32 when tested against normal Cat serum in agar block titration.
Reactivities Feline
Conjugation Unconjugated

Feline IgA (Fc specific) rabbit polyclonal antibody, Serum

Applications In precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgA in Cat serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of cat IgA from serum or plasma.
Immunoelectrophoresis: Use 2 µl serum or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl serum samples (neat and serially) diluted in 2 mm diameter peripheral wells.
Antibody titre: 1/32 (Precipitin titre) when tested against pooled normal Cat serum in agar-block immunodiffusion titration.
Reactivities Feline
Conjugation Unconjugated

Feline IgA mouse monoclonal antibody, clone IgA9-6A, Purified

Applications Western Blot.
ELISA: This antibody may be used as a capture antibody in an ELISA in combination with either SM1503B or AM01151BT-N as detecting antibody.
Reactivities Feline
Conjugation Unconjugated

Feline IgG (Fc specific) goat polyclonal antibody, FITC

Applications Flow Cytometry.
Immunohistochemistry on Frozen Sections: 1/200-1/2,000.
Reactivities Feline
Conjugation FITC

Feline IgG (Fc specific) goat polyclonal antibody, Aff - Purified

Applications ELISA: 1/100-1/10,00.
Immunodiffusion.
Reactivities Feline
Conjugation Unconjugated

Feline IgM goat polyclonal antibody, FITC

Applications Flow Cytometry.
Immunohistochemistry on Frozen Sections.
Reactivities Feline
Conjugation FITC

Feline IgM goat polyclonal antibody, HRP

Applications ELISA: 1/10,000 - 1/100,000.
Reactivities Feline
Conjugation HRP

Monkey IgA mouse monoclonal antibody, clone IgA5-3B, Biotin

Applications ELISA: This antibody may be used in a Direct ELISA or as a Detection antibody in a Sandwich ELISA together with product code SM1508P as a Capture reagent.
Reactivities Canine, Feline, Human, Monkey
Conjugation Biotin