Secondary Antibodies

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Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, HRP

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay.
Reactivities Human
Conjugation HRP

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, Purified

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay
Reactivities Human
Conjugation Unconjugated

Human IgA (Secretory component) mouse monoclonal antibody, clone NI 194-4 (A89-039), HRP

Applications To identify the presence of free or bound Secretory component in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: from 1/20 upwards.  
ELISA: form 1/50 upwards.  
Western blot: from 1/100 upwards.
Reactivities Human
Conjugation HRP

Human IgA (IgAc, A1, A2, Secretory) mouse monoclonal antibody, clone NI 69 and NI 184+NI 69-11+NI 512+NI 194, Ascites

Applications Can be used to identify the presence of IgA and secretory subclasses in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radioimmunoassay, ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgA, subclasses or secretory component and immunoblotting. 
Recommended Working Dilutions:
Histochemical Use: 1/100-1/500.
ELISA: from 1/1000 Upwards. 
Western blotting: 1/2000-1/10000.
Reactivities Human
Conjugation Unconjugated

Human IgG, IgA, IgM, IgD, IgE mouse monoclonal antibody, clone NI 335 and NI 343, NI 69 and NI 184, NI 179, NI 158, NI 307, Ascites

Applications Can be used to identify the presence of immunoglobulin classes in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radioimmunoassay, ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG, subclasses and immunoblotting.
Recommended Working Dilutions:
Histochemical Use: 1/100-1/500.
ELISA: from 1/1000 Upwards.
Western blotting: 1/2000 Upwards.
Reactivities Human
Conjugation Unconjugated

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, Biotin

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay
Reactivities Human
Conjugation Biotin

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, FITC

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay
Reactivities Human
Conjugation FITC

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, PE

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay
Reactivities Human
Conjugation PE

Feline IgG (H+L chain) goat polyclonal antibody, Serum

Applications Can be used:
• In precipitating techniques as Immunoelectrophoresis and Double Radial Immunodiffusion to identify the presence of IgG.
• As secondary antibody to precipitate the immunoglobulin in normal Cat serum.
• To prepare an immunoadsorbent for the production of Ig-free Cat serum, plasma or non-Ig preparations.
Directions for use:
Immunoelectrophoresis: Use 2 μl or equivalent against 120 μl antiserum. 
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Reactivities Feline
Conjugation Unconjugated