Secondary Antibodies

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IGHG4 mouse monoclonal antibody, clone HP6025, AP, Purified

Applications ELISA: 1/1000-1/2000 (2-9).
Immunohistochemistry on Frozen Sections:  reported in literature (5,7,10,11).
Immunohistochemistry on Paraffin Sections: reported in literature (10).
Flow Cytometry:
reported in literature (15, 18, 19).
Immunocytochemistry: reported in literature (12).
Western Blot: reported in literature (3,13-16).
Reactivities Human
Conjugation AP

FITC Conjugated Goat Anti-Rabbit IgG

Applications FC: 1:100; IF/ICC: 1:32-64; IHC: 1:32-64
Reactivities Rabbit IgG
Conjugation FITC

Mouse IgG2a (heavy chain) rat monoclonal antibody, clone LO-MG2a-7, FITC

Applications Flow Cytometry: Use 10 µl of 2.5 µg/ml diluted antibody to label 106 cells in 100 µl.
Reactivities Mouse
Conjugation FITC

Bovine IgG (light chain) mouse monoclonal antibody, clone IVA285-1, Purified

Applications Western Blotting (Reducing Conditions): 0.5-1 μg/ml, overnight in 4°C.
SDS-PAGE (12% separating gel).
Positive Control and Sample Peparation: BS (Bovine Serum), dilution 1/50 in Laemmli reducing buffer, boiled in water bath for 3 min.
Strongly reacts with Bovine IgG light chains, weakly reacts with IgM.
Flow Cytometry.
Immunohistochemistry on Frozen Sections.
Reactivities Bovine, Sheep
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone BE5, Purified

Applications ELISA.
Flow Cytometry: 5-10 µg/ml.
Reactivities Human
Conjugation Unconjugated

Human IgM (Fc-region) mouse monoclonal antibody, clone CH2, Purified

Applications Western blot. Recommended dilution: 1 μg/ml.
Flow cytometry: Recommended dilution: 1-4 μg/ml, extracellular and intracellular staining.
Immunocytochemistry.
ELISA.
Reactivities Human
Conjugation Unconjugated

Rat IgM (Mu chain specific) mouse monoclonal antibody, clone M 2A1, PE

Applications Flow Cytometry: (< / = 0.1 µg/10e6 cells) and Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells.
FLISA: < / = 1.0 µg/ml
Reactivities Rat
Conjugation PE

Mouse IgA (alpha chain specific) goat polyclonal antibody, Biotin

Applications

ELISA: 1/5,000-1/20,000.
Immunoblotting. 
Flow Cytometry.

Immunohistochemistry.

Reactivities Mouse
Conjugation Biotin

Human IgM (Fc-region) mouse monoclonal antibody, clone CH2, FITC

Applications Flow cytometry (1/500 starting dilution).
Reactivities Human
Conjugation FITC

Human IgM (Fc-region) mouse monoclonal antibody, clone CH2, APC

Applications Flow Cytometry: 2 µg/ml is recommended starting point.
Reactivities Human
Conjugation APC

Human IgM (Fc specific) mouse monoclonal antibody, clone CH2, PE

Applications Flow cytometry: 0.5 µg/ml.
Reactivities Human
Conjugation PE

Mouse IgG (H+L chain), adsorbed goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 50µl of 1/50-1/200 diluted antibody to label 106 cells in 100 µl.
Reactivities Mouse
Conjugation FITC

Human IgE mouse monoclonal antibody, clone BE5, FITC

Applications Flow cytometry: 5 µg/ml.
Reactivities Human
Conjugation FITC

Human IgE mouse monoclonal antibody, clone BE5, PE

Applications Flow cytometry: 5 µg/ml.
Reactivities Human
Conjugation PE

FITC Conjugated Goat Anti-Mouse IgG

Applications FC: 1:100; IF/ICC: 1:32-64; IHC: 1:32-64
Reactivities Mouse IgG
Conjugation FITC