E. coli Selection
This Lenti vector is a third generation lentiviral vector.
All OriGene's TrueORF clones can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process. The ORF (no stop codon for P2A-tGFP) cloned in this vector will be expressed as native protein, no tag. P2A-tGFP is within the Lentiviral packaging cassette and would allow GFP to enrich both the transfected and the transduced cells. A brief lentiviral packaging protocol is provided. E. Coli. selection: Chloramphemicol (34 ug/ml) Download full vector sequence
Schematic of the multiple cloning sites
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