pLenti-EF1a-N-HA-C-Myc-DDK-IRES-Puro Tagged Cloning Vector
pLenti-EF1a-N-HA-C-Myc-DDK-IRES-Puro, Lenti vector with EF1a promoter, N-terminal HA tag, C-terminal Myc-DDK tag and IRES-Puro, 10 ug
E. coli Selection
This Lenti vector is a third generation lentiviral vector.
All OriGene's TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process. The expression of the cloned ORF is driven by EF1a, a constitutive promoter as an alternative of CMV promoter. The latter could be silenced in certain cellular environment. The ORF cloned in this vector can be expressed as a tagged protein with N-terminal HA tag and C-terminal Myc-DDK tag. IRES-Puro is within the Lentiviral packaging cassette and would allow puromycin selection in both the transfected and the transduced cells. A brief lentiviral packaging protocol is provided. E. Coli. selection: Chloramphemicol (34 ug/ml) Download full vector sequence
Schematic of the multiple cloning sites
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.