pLenti-EF1a-N-HA-C-Myc-DDK-IRES-Puro Tagged Cloning Vector

CAT#: PS100106

Product images

Specifications

Product Data

• C-Tag: Myc-DDK
• N-Tag: HA
• E. coli Selection: Chloramphenicol
• Cell Selection: Puromycin
• Features:

  This Lenti vector is a third generation lentiviral vector.

  • All OriGene's TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process.
  • The expression of the cloned ORF is driven by EF1a, a constitutive promoter as an alternative of CMV promoter. The latter could be silenced in certain cellular environment.
  • The ORF cloned in this vector can be expressed as a tagged protein with N-terminal HA tag and C-terminal Myc-DDK tag.
  • IRES-Puro is within the Lentiviral packaging cassette and would allow puromycin selection in both the transfected and the transduced cells.
  • A brief lentiviral packaging protocol is provided.
  • E. Coli. selection: Chloramphemicol (34 ug/ml)
  • Download full vector sequence
• Schematic of the multiple cloning sites:

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.