pLenti-EF1a-C-Myc-DDK-IRES-Puro      Tagged Cloning Vector

CAT#: PS100085

pLenti-EF1a-C-Myc-DDK-IRES-Puro , Lenti Vector with EF1 alpha promoter and bicistronic IRES-Puro  , 10ug

USD 732


Availability*
In Stock

Size
    • 10 ug

Product images

Specifications

Product Data
C-Tag Myc-DDK
E. coli Selection Chloramphenicol
Cell Selection Puromycin
Features

This Lenti vector is a third generation of lentiviral vector.

  • All OriGene’s TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process.
  • The expression of the cloned ORF is driven by EF1a, a constitutive promoter as an alternative of CMV promoter. The latter could be silenced in certain cellular environment.
  • The ORF cloned in this vector can be expressed as a tagged protein with c-terminal Myc-DDK tags.
  • IRES-Puro is within the Lentiviral packaging cassette and would allow Puromycin selection in both the transfected and the transduced cells.
  • A brief lentiviral packaging protocol is provided
  • Download full vector sequence
Schematic of the multiple cloning sites
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations