pLenti-EF1a-C-mGFP Lentiviral Gene Expression Vector

CAT#: PS100084

pLenti-EF1a-C-mGFP, Lenti Vector with EF1 alpha promoter and C-terminal mGFP tag, 10ug

Product Images

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Specifications

Product Data

• C-Tag: mGFP
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Features:

  This Lenti vector is a third generation of lentiviral vector.

  • All OriGene’s TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process.
  • The ORF cloned in this vector can be expressed as a tagged protein with a C-terminal mGFP tag.
  • The expression of the cloned ORF is driven by EF1a, a constitutive promoter as an alternative of CMV promoter. The latter could be silenced in certain cellular environment.
  • A brief lentiviral packaging protocol is provided.
  E. Coli selection: Chloramphenicol(34ug/ml)
  • EF51 Primer can be used to sequence the target sequence after cloning.
  • mGFP_R Primer can be used to sequence the target sequence after cloning.
• Schematic of the multiple cloning sites: