E. coli Selection
ORFs cloned in this vector will be expressed in mammalian cells as fusion proteins with 3 tandem DDK-tags on the C-terminal ends, resulting in 100X higher detection sensitivity than 1XDDK tag The 3DDK tag will increase the affinity of the fusion protein to anti-DDK tags and facilitate detection. This is especially useful for proteins of low expression level. If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal 3DDK-tagging vector, PS100058. Download full vector sequence
Schematic of the multiple cloning sites
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