ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with a C-terminal mBFP (monomeric) tag.
mBFP (scientific name mTagBFP) is a monomeric blue fluorescent protein. mBFP possesses bright blue fluorescence with excitation/emission maxima at 402 and 457 nm, characterized by high photostability and extremely high pH-stability. Compared to EBFP2, mBFP is more than 1.8 times brighter, much more pH-stable and has twice shorter maturation half-time at 37°C. Narrow fluorescence emission peak of mBFP provides for accurate and easy spectral separation with cyan and green fluorescent proteins and makes it a preferable tag for multicolor labeling. Good overlap between the emission spectrum of mBFP and the absorbance spectra of mGFP allows using these two proteins as a FRET pair.
If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal mBFP-tagging vector, PS100051.
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