ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with an N-terminal tYFP tag.
TurboYFP (excitation/ emission max = 525/ 538nm) is an enhanced variant of the yellow fluorescent protein PhiYFP . TurboYFP is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell labeling and tracking the promoter activity. Structually it forms dimer. Learn more about TurboYFP
Such clones are the best for monitoring protein expression, localization, translocation and co-localization with other protein(s).
If the N-terminal tagging interferes with the protein’s function, you can choose the C-terminal tYFP-tagging vector, PS100036
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